Detailed information NTG0372213

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FNA74_RS04680
• Genome accession: NZ_CP041515
• Coordinates: 729584..730381 (-)
• Length: 265 a.a.
• NCBI ID: WP_064374757.1
• Uniprot ID: A0A7H4LZS4
• Organism: Klebsiella michiganensis strain KNU07
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 724584..735381

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNA74_RS04660	dksA	725023..725478 (-)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-
FNA74_RS04665	sfsA	725643..726359 (-)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
FNA74_RS04670	thpR	726359..726895 (-)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
FNA74_RS04675	hrpB	726968..729397 (+)	2430	WP_064374758.1	ATP-dependent helicase HrpB	-
FNA74_RS04680	pilD	729584..730381 (-)	798	WP_064374757.1	prepilin peptidase	Machinery gene
FNA74_RS04685	-	730384..731142 (-)	759	WP_064374756.1	type II secretion system protein N	-
FNA74_RS04690	gspM	731132..731617 (-)	486	WP_025107362.1	type II secretion system protein GspM	-
FNA74_RS04695	gspL	731610..732803 (-)	1194	WP_064374754.1	type II secretion system protein GspL	-
FNA74_RS04700	gspK	732796..733776 (-)	981	WP_064374753.1	type II secretion system minor pseudopilin GspK	-
FNA74_RS04705	gspJ	733773..734369 (-)	597	WP_047724421.1	type II secretion system minor pseudopilin GspJ	-
FNA74_RS04710	gspI	734366..734731 (-)	366	WP_048262356.1	type II secretion system minor pseudopilin GspI	-
FNA74_RS04715	gspH	734728..735240 (-)	513	WP_049083165.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29180.80 Da
• Isoelectric Point: 8.3358

>NTDB_id=372213 FNA74_RS04680 WP_064374757.1 729584..730381(-) (pilD) [Klebsiella michiganensis strain KNU07]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAIIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=372213 FNA74_RS04680 WP_064374757.1 729584..730381(-) (pilD) [Klebsiella michiganensis strain KNU07]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGATCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A7H4LZS4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.339	100	0.494
pilD	Neisseria gonorrhoeae MS11	44.245	100	0.464
pilD	Vibrio campbellii strain DS40M4	44	100	0.457
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438