Detailed information NTG0371808

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E2649_RS23770
• Genome accession: NZ_CP041390
• Coordinates: 4461817..4462614 (+)
• Length: 265 a.a.
• NCBI ID: WP_004858028.1
• Uniprot ID: -
• Organism: Raoultella ornithinolytica strain 2015ndm
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4456817..4467614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E2649_RS23735	gspH	4456937..4457455 (+)	519	WP_123629631.1	type II secretion system minor pseudopilin GspH	-
E2649_RS23740	gspI	4457452..4457817 (+)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
E2649_RS23745	gspJ	4457829..4458413 (+)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
E2649_RS23750	gspK	4458410..4459396 (+)	987	WP_054829280.1	type II secretion system minor pseudopilin GspK	-
E2649_RS23755	gspL	4459383..4460585 (+)	1203	WP_004858036.1	type II secretion system protein GspL	-
E2649_RS23760	-	4460578..4461072 (+)	495	WP_123629630.1	type II secretion system protein M	-
E2649_RS23765	-	4461062..4461802 (+)	741	WP_032716580.1	type II secretion system protein N	-
E2649_RS23770	pilD	4461817..4462614 (+)	798	WP_004858028.1	A24 family peptidase	Machinery gene
E2649_RS23775	hrpB	4462635..4465064 (-)	2430	WP_123629629.1	ATP-dependent helicase HrpB	-
E2649_RS23780	thpR	4465137..4465673 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
E2649_RS23785	sfsA	4465673..4466389 (+)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
E2649_RS23790	dksA	4466554..4467009 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29315.95 Da
• Isoelectric Point: 8.4762

>NTDB_id=371808 E2649_RS23770 WP_004858028.1 4461817..4462614(+) (pilD) [Raoultella ornithinolytica strain 2015ndm]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLTGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=371808 E2649_RS23770 WP_004858028.1 4461817..4462614(+) (pilD) [Raoultella ornithinolytica strain 2015ndm]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GACCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGCGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	44.574	97.358	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43