Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FLG15_RS14680 Genome accession   NZ_CP041380
Coordinates   3248737..3249774 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas phaseoli pv. dieffenbachiae strain D182     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243737..3254774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLG15_RS14660 (FLG15_14670) - 3244622..3245194 (+) 573 WP_057679629.1 DNA-3-methyladenine glycosylase I -
  FLG15_RS14665 (FLG15_14675) - 3245202..3246137 (+) 936 WP_170922402.1 DUF72 domain-containing protein -
  FLG15_RS14670 (FLG15_14680) - 3246304..3246978 (-) 675 WP_057679636.1 YitT family protein -
  FLG15_RS14675 (FLG15_14685) pilU 3247484..3248614 (-) 1131 WP_008571827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FLG15_RS14680 (FLG15_14690) pilT 3248737..3249774 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  FLG15_RS14685 (FLG15_14695) - 3250192..3250884 (+) 693 WP_057679627.1 YggS family pyridoxal phosphate-dependent enzyme -
  FLG15_RS14690 (FLG15_14700) proC 3250928..3251782 (+) 855 WP_057679626.1 pyrroline-5-carboxylate reductase -
  FLG15_RS14695 (FLG15_14705) - 3252346..3252771 (+) 426 WP_017159922.1 HU family DNA-binding protein -
  FLG15_RS14700 (FLG15_14710) - 3252874..3253515 (+) 642 WP_057679625.1 M23 family metallopeptidase -
  FLG15_RS14705 (FLG15_14715) - 3253573..3253914 (+) 342 WP_003489901.1 DUF3861 family protein -
  FLG15_RS14710 (FLG15_14720) - 3254157..3254513 (+) 357 WP_081022750.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=371746 FLG15_RS14680 WP_003489919.1 3248737..3249774(-) (pilT) [Xanthomonas phaseoli pv. dieffenbachiae strain D182]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=371746 FLG15_RS14680 WP_003489919.1 3248737..3249774(-) (pilT) [Xanthomonas phaseoli pv. dieffenbachiae strain D182]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCGCTGGACCATAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAGGTCGACTTTTCGTTCGAGATTCCGTCGCTGGCGCGT
TTTCGCGTCAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGTTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAATGAGTACGGGCATATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCTTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGTTTCAACGA
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTCACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATTTCGCAGGC
GCTGTTGAAGAAGGTCGGCGGCGGCCGTACGGCGGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGATCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment