Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FLK62_RS02535 Genome accession   NZ_CP041334
Coordinates   513783..514364 (-) Length   193 a.a.
NCBI ID   WP_010786056.1    Uniprot ID   A0A859IE21
Organism   Glaesserella parasuis strain HPS412     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508783..519364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLK62_RS02495 (FLK62_02515) - 509131..509769 (+) 639 WP_176443450.1 nitroreductase family protein -
  FLK62_RS02500 (FLK62_02520) - 509858..510391 (+) 534 WP_015940082.1 TIGR00645 family protein -
  FLK62_RS02505 (FLK62_02525) - 510476..511774 (-) 1299 WP_075605717.1 FAD-binding oxidoreductase -
  FLK62_RS02525 (FLK62_02545) greB 512308..512787 (-) 480 WP_071610788.1 transcription elongation factor GreB -
  FLK62_RS02530 (FLK62_02550) - 512787..513722 (-) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  FLK62_RS02535 (FLK62_02555) pilF 513783..514364 (-) 582 WP_010786056.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FLK62_RS02540 (FLK62_02560) - 514412..515383 (-) 972 WP_012621529.1 IS110 family transposase -
  FLK62_RS02545 (FLK62_02565) - 515619..516779 (-) 1161 WP_176443451.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FLK62_RS02550 (FLK62_02570) - 516980..517207 (-) 228 WP_016528332.1 hypothetical protein -
  FLK62_RS02555 (FLK62_02575) - 517313..517549 (-) 237 WP_015940086.1 hypothetical protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.29 Da        Isoelectric Point: 8.4221

>NTDB_id=371270 FLK62_RS02535 WP_010786056.1 513783..514364(-) (pilF) [Glaesserella parasuis strain HPS412]
MTFAKFFRNLTACSVALWLVACTNQTAKVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=371270 FLK62_RS02535 WP_010786056.1 513783..514364(-) (pilF) [Glaesserella parasuis strain HPS412]
ATGACCTTCGCAAAATTTTTCCGTAATTTGACCGCTTGTAGTGTGGCGTTATGGCTTGTTGCTTGTACAAATCAAACCGC
AAAGGTGGATTTTAACCGTTCAGAAGCCGTCAAAGCTCGTATTAATTTGGCTCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A859IE21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

97.927

100

0.979
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.251

92.746

0.42

Multiple sequence alignment