Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3273_RS19260 Genome accession   NZ_CP048647
Coordinates   4020388..4020828 (+) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain GW-AmxH19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4015388..4025828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3273_RS19240 (G3273_19240) aroP 4016388..4017758 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  G3273_RS19245 (G3273_19245) ampE 4017801..4018655 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  G3273_RS19250 (G3273_19250) ampD 4018652..4019203 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G3273_RS19255 (G3273_19255) nadC 4019291..4020184 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G3273_RS19260 (G3273_19260) pilA 4020388..4020828 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  G3273_RS19265 (G3273_19265) pilB 4020838..4022223 (+) 1386 WP_001535814.1 type II secretion system protein GspE Machinery gene
  G3273_RS19270 (G3273_19270) hofC 4022213..4023415 (+) 1203 WP_000157243.1 protein transport protein HofC -
  G3273_RS19275 (G3273_19275) guaC 4023450..4024493 (-) 1044 WP_001217338.1 GMP reductase -
  G3273_RS24855 - 4024649..4024693 (-) 45 WP_120795372.1 protein YacM -
  G3273_RS19280 (G3273_19280) coaE 4024718..4025338 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=370272 G3273_RS19260 WP_000360913.1 4020388..4020828(+) (pilA) [Escherichia coli strain GW-AmxH19]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=370272 G3273_RS19260 WP_000360913.1 4020388..4020828(+) (pilA) [Escherichia coli strain GW-AmxH19]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363