Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D073_RS12110 Genome accession   NZ_CP041145
Coordinates   2492312..2492926 (-) Length   204 a.a.
NCBI ID   WP_020956097.1    Uniprot ID   -
Organism   Bacillus velezensis strain At1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2487312..2497926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D073_RS12080 (D073_2292) rpsT 2487615..2487881 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  D073_RS12085 (D073_2293) holA 2487898..2488939 (-) 1042 Protein_2320 DNA polymerase III subunit delta -
  D073_RS12090 - 2488974..2489130 (-) 157 Protein_2321 hypothetical protein -
  D073_RS12095 - 2489171..2489305 (+) 135 WP_003152870.1 YqzM family protein -
  D073_RS12100 (D073_2294) comEC 2489324..2491675 (-) 2352 WP_032866696.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  D073_RS12105 (D073_2295) - 2491676..2492245 (-) 570 WP_007408264.1 ComE operon protein 2 -
  D073_RS12110 (D073_2296) comEA 2492312..2492926 (-) 615 WP_020956097.1 helix-hairpin-helix domain-containing protein Machinery gene
  D073_RS12115 (D073_2297) comER 2492985..2493806 (+) 822 WP_020956098.1 late competence protein ComER -
  D073_RS12120 (D073_2298) - 2493876..2494613 (-) 738 WP_015240247.1 class I SAM-dependent DNA methyltransferase -
  D073_RS12125 (D073_2299) rsfS 2494610..2494966 (-) 357 WP_007408260.1 ribosome silencing factor -
  D073_RS12130 (D073_2300) yqeK 2494984..2495544 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D073_RS12135 (D073_2301) - 2495534..2496103 (-) 570 WP_032866687.1 nicotinate-nucleotide adenylyltransferase -
  D073_RS12140 (D073_2302) yhbY 2496114..2496404 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  D073_RS12145 (D073_2303) aroE 2496398..2497240 (-) 843 WP_007408256.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22251.45 Da        Isoelectric Point: 6.4940

>NTDB_id=369835 D073_RS12110 WP_020956097.1 2492312..2492926(-) (comEA) [Bacillus velezensis strain At1]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=369835 D073_RS12110 WP_020956097.1 2492312..2492926(-) (comEA) [Bacillus velezensis strain At1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACCGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363

Multiple sequence alignment