Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   FEE42_RS04030 Genome accession   NZ_CP040863
Coordinates   829180..829725 (-) Length   181 a.a.
NCBI ID   WP_164027766.1    Uniprot ID   -
Organism   Rodentibacter caecimuris strain G1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824180..834725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEE42_RS04005 (FEE42_03985) - 824620..825063 (-) 444 WP_164027762.1 methyltransferase family protein -
  FEE42_RS04010 (FEE42_03990) - 825093..825707 (-) 615 WP_164027763.1 YfgM family protein -
  FEE42_RS04015 (FEE42_03995) hisS 825726..826997 (-) 1272 WP_164027764.1 histidine--tRNA ligase -
  FEE42_RS04020 (FEE42_04000) ispG 827007..828113 (-) 1107 WP_164027765.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FEE42_RS04025 (FEE42_04005) - 828126..829088 (-) 963 WP_237184476.1 RodZ domain-containing protein -
  FEE42_RS04030 (FEE42_04010) pilF/pilF2 829180..829725 (-) 546 WP_164027766.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FEE42_RS04035 (FEE42_04015) - 829751..830905 (-) 1155 WP_135969346.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FEE42_RS04040 (FEE42_04020) - 831117..832001 (+) 885 WP_164027767.1 D-alanyl-D-alanine carboxypeptidase family protein -
  FEE42_RS04045 (FEE42_04025) - 832250..833131 (+) 882 WP_059365609.1 metal ABC transporter substrate-binding protein -
  FEE42_RS04050 (FEE42_04030) - 833131..834036 (+) 906 WP_059365610.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20669.45 Da        Isoelectric Point: 7.8106

>NTDB_id=367717 FEE42_RS04030 WP_164027766.1 829180..829725(-) (pilF/pilF2) [Rodentibacter caecimuris strain G1]
MKPLFIRFLSAVIFPLVFSACVSQAPSSDFNKQQAAKARVELALGYLQQNNFIQAKQNLDKALAHDQEYYLVHSAFAHFH
QLQGDVNEARKSYLKSIKLDEKQGDVHNNFGAFLCSQGEFEQANKQFELALLSPNYYRQADTYENMVLCSQALNQSEAYQ
KAIDKLRQIDVNRAEKLSLIK

Nucleotide


Download         Length: 546 bp        

>NTDB_id=367717 FEE42_RS04030 WP_164027766.1 829180..829725(-) (pilF/pilF2) [Rodentibacter caecimuris strain G1]
ATGAAACCTTTATTTATTCGATTTTTAAGTGCGGTCATTTTTCCTCTTGTTTTTTCAGCTTGTGTTTCGCAAGCCCCCTC
TTCTGATTTTAATAAACAACAGGCGGCAAAAGCGCGAGTCGAACTGGCGCTTGGCTATTTGCAGCAAAATAATTTTATTC
AAGCGAAACAAAATCTTGATAAAGCGTTAGCACATGATCAAGAGTATTATCTTGTTCATTCTGCCTTTGCACATTTTCAT
CAATTGCAAGGCGATGTTAATGAAGCAAGAAAATCTTACCTTAAATCCATAAAACTTGATGAAAAACAAGGTGATGTACA
TAATAATTTTGGCGCGTTTTTATGTAGTCAAGGTGAGTTTGAACAAGCCAATAAGCAATTTGAACTTGCCTTACTTTCGC
CTAATTATTACCGTCAGGCAGATACTTATGAAAATATGGTGCTTTGCTCACAGGCGCTAAATCAATCGGAGGCTTATCAA
AAAGCAATCGACAAATTACGACAAATAGATGTGAATCGTGCGGAAAAACTAAGTCTAATCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(77-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.162

95.58

0.613
  pilF Glaesserella parasuis strain SC1401

44.199

100

0.442

Multiple sequence alignment