Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MRUB_RS13755 Genome accession   NC_013946
Coordinates   2778345..2779481 (+) Length   378 a.a.
NCBI ID   WP_013014985.1    Uniprot ID   -
Organism   Meiothermus ruber DSM 1279     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2773345..2784481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRUB_RS13745 (Mrub_2737) - 2775518..2776981 (+) 1464 WP_013014983.1 lyase family protein -
  MRUB_RS13750 (Mrub_2738) - 2777142..2778149 (+) 1008 WP_013014984.1 isocitrate/isopropylmalate dehydrogenase family protein -
  MRUB_RS13755 (Mrub_2739) pilM 2778345..2779481 (+) 1137 WP_013014985.1 type IV pilus assembly protein PilM Machinery gene
  MRUB_RS13760 (Mrub_2740) pilN 2779474..2780139 (+) 666 WP_013014986.1 competence protein Machinery gene
  MRUB_RS13765 (Mrub_2741) pilO 2780141..2780737 (+) 597 WP_013014987.1 type 4a pilus biogenesis protein PilO -
  MRUB_RS13770 (Mrub_2742) - 2780734..2781759 (+) 1026 WP_013014988.1 hypothetical protein -
  MRUB_RS13775 (Mrub_2743) - 2781769..2783298 (+) 1530 WP_013014989.1 secretin N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41355.78 Da        Isoelectric Point: 4.5051

>NTDB_id=36687 MRUB_RS13755 WP_013014985.1 2778345..2779481(+) (pilM) [Meiothermus ruber DSM 1279]
MRDFFGGLLRPRIEALGLEIGSANIKMVELSGTPPSLKNLSIRPTPPGVVQEGSIIEPGALTEVIKEMLAEMRTRKRYVV
TAASNLAVITRTLQVPKMPIKQMEEAVRWEAERYIPFPIDEVVLDYAPLDSLEAIPDGEQMDVVVGAARQETVASLVEAL
KAAGLEPLVIDVKPFAGLRTLGARLAATDREPITLFLEIGAESSALVLTRGERLLLNRVINLSGKDFTAAISKAFNLDVV
AAEDAKKNYGLATIPTEDEDLLLDFDAERERFNPARMYDAIRPVLVELTTELRRSLEFFRVQVGDLGVDQGFVAGGGSKL
RSLVPLLSDTLGIPLETADPWQGLQIDKSRFDLEYLRSLAPEFTVPVGLALRGVNPLD

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=36687 MRUB_RS13755 WP_013014985.1 2778345..2779481(+) (pilM) [Meiothermus ruber DSM 1279]
GTGCGTGATTTCTTTGGCGGGTTGCTGAGACCCAGAATCGAGGCGCTCGGCCTCGAGATTGGATCGGCCAATATCAAAAT
GGTCGAGCTGTCCGGAACTCCCCCCAGCCTGAAAAACCTGAGCATCAGGCCCACCCCGCCAGGGGTGGTTCAGGAGGGTT
CGATCATCGAGCCGGGCGCCCTGACCGAGGTCATCAAAGAGATGCTGGCTGAGATGCGCACCCGCAAGCGCTACGTGGTA
ACGGCGGCCAGCAACCTGGCGGTCATTACCCGCACCTTGCAGGTGCCCAAGATGCCCATCAAACAGATGGAGGAAGCTGT
ACGCTGGGAAGCTGAACGCTACATCCCTTTCCCAATTGACGAGGTGGTGCTGGATTACGCCCCCCTCGACTCCCTTGAGG
CCATTCCAGATGGGGAGCAGATGGACGTGGTGGTAGGGGCGGCCCGGCAGGAGACCGTGGCCAGCCTGGTCGAGGCCCTC
AAAGCCGCCGGTCTGGAGCCATTGGTGATCGACGTTAAGCCATTTGCCGGTCTGCGCACCCTGGGCGCCCGCCTGGCGGC
CACCGACCGTGAGCCCATCACCCTCTTCCTCGAGATCGGGGCTGAATCCTCGGCCCTGGTTCTGACCCGTGGTGAGCGGT
TGCTCCTGAACCGCGTCATCAACCTATCCGGTAAGGACTTCACCGCTGCGATTTCGAAAGCGTTCAACCTCGATGTGGTG
GCCGCCGAAGACGCCAAGAAAAACTACGGCCTGGCTACCATCCCCACCGAAGATGAAGACCTGCTGCTCGACTTCGACGC
CGAGCGCGAGCGCTTCAATCCGGCCCGTATGTACGACGCCATCCGCCCGGTGCTGGTCGAGCTGACCACCGAGCTGCGCC
GAAGCCTGGAGTTCTTCCGGGTTCAGGTGGGCGACCTGGGGGTCGATCAGGGCTTTGTGGCCGGAGGGGGCAGCAAGCTA
AGAAGCTTGGTGCCGCTTTTGTCTGATACACTGGGCATCCCACTGGAAACCGCCGACCCCTGGCAGGGTCTTCAAATCGA
TAAAAGCCGCTTCGATCTGGAGTACCTGCGCAGTCTGGCGCCCGAGTTTACGGTACCGGTGGGCCTGGCCCTACGGGGGG
TGAATCCGCTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

64.247

98.413

0.632

Multiple sequence alignment