Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FFX45_RS02405 Genome accession   NZ_CP040671
Coordinates   485862..487886 (+) Length   674 a.a.
NCBI ID   WP_149817849.1    Uniprot ID   -
Organism   Thermosynechococcus sp. CL-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480862..492886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFX45_RS02375 (FFX45_02375) leuD 481078..481668 (+) 591 WP_149817841.1 3-isopropylmalate dehydratase small subunit -
  FFX45_RS02380 (FFX45_02380) - 481661..482617 (-) 957 WP_190278172.1 glycosyltransferase family 2 protein -
  FFX45_RS02385 (FFX45_02385) - 482708..483670 (+) 963 WP_149817843.1 hypothetical protein -
  FFX45_RS02390 (FFX45_02390) - 483667..485142 (-) 1476 WP_190278173.1 right-handed parallel beta-helix repeat-containing protein -
  FFX45_RS02395 (FFX45_02395) - 485190..485387 (-) 198 WP_024125597.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  FFX45_RS02400 (FFX45_02400) - 485388..485600 (-) 213 WP_149817847.1 hypothetical protein -
  FFX45_RS02405 (FFX45_02405) pilB/pilB1 485862..487886 (+) 2025 WP_149817849.1 GspE/PulE family protein Machinery gene
  FFX45_RS02410 (FFX45_02410) pilT 487954..489051 (+) 1098 WP_149817851.1 type IV pilus twitching motility protein PilT Machinery gene
  FFX45_RS13015 - 489088..490301 (+) 1214 Protein_480 type II secretion system F family protein -
  FFX45_RS02420 (FFX45_02420) - 490310..490783 (+) 474 WP_149817853.1 J domain-containing protein -
  FFX45_RS02425 (FFX45_02425) - 490780..491052 (+) 273 WP_149817855.1 DUF3143 domain-containing protein -
  FFX45_RS02430 (FFX45_02430) - 491049..491402 (-) 354 WP_149817857.1 hypothetical protein -
  FFX45_RS02435 (FFX45_02435) - 491697..492842 (-) 1146 WP_190278174.1 cysteine desulfurase family protein -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74306.57 Da        Isoelectric Point: 5.5500

>NTDB_id=366313 FFX45_RS02405 WP_149817849.1 485862..487886(+) (pilB/pilB1) [Thermosynechococcus sp. CL-1]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGSTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTAGKNAPAAIGDINIEEDIEAMGGLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVAR
LSEMGVEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKASGQPICS
KCGGVGYKGRVGVYEIMRVTERLQSLITEGAPTERIKEAAVEEGMKTLLAYSLNLVKEGVTTLEEVERVTFTDTGLESEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=366313 FFX45_RS02405 WP_149817849.1 485862..487886(+) (pilB/pilB1) [Thermosynechococcus sp. CL-1]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGAGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTACAGGAGATTACCGGCAGCACCATGCCCCCCGATGTGTTGCGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGCGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTAATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACTGC
TGGCAAAAACGCCCCGGCCGCGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCGGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCCGAGGCGCTGAAAGGCGCAGAAGATGCTCCGATTATTGCCCTTGTCAACAAAATT
TTGGCCAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTCCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCCGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGAATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCAGCGCTGGCTGAATGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCCATTCTGCGTGCCTTTCTCCGCCAAGACCCCGATGTGATTCTGGTGGGGGAAACCCGCGACAAGGAAACAGCAAAAA
CCGCTATTGAAGCTGCCTTGACAGGTCACTTGGTGTTAACCACCCTGCACACCAACGATGCCGCCAGTGCCGTTGCCCGT
CTCTCGGAAATGGGGGTGGAACCCTTCATGGTCTCCGCTTCCTTAATTGGGGTCGTTGCCCAGCGACTTATGCGGCGGGT
ATGCAGTGAGTGTCGCATTCCCTATACGCCCACCCGTGAGGAACTGGCACGCTTTGGCTTGTCTGCCTCCAAGGATGTCA
ACCTCACCCTCTACAAAGCCAATAAACTCACACCCGAGCAGATTCAAGCAGCCAAAGCCAGTGGCCAGCCGATTTGTAGC
AAGTGTGGCGGTGTTGGCTACAAAGGACGGGTCGGGGTCTACGAAATTATGCGGGTGACGGAGCGCCTGCAAAGCCTGAT
TACTGAGGGTGCCCCCACGGAGCGGATCAAGGAAGCGGCGGTTGAAGAGGGCATGAAAACCCTGTTGGCCTATAGCCTGA
ACCTTGTCAAAGAAGGGGTCACCACCCTCGAGGAAGTCGAGCGCGTCACCTTTACGGACACGGGTCTCGAATCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)

Type II/IV secretion system protein (T2SSE)

(239-506)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.549

97.329

0.638
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.148

94.51

0.361

Multiple sequence alignment