Detailed information NTG0366151

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E3148_RS21450
• Genome accession: NZ_CP040657
• Coordinates: 4415651..4416451 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 2019010306
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4410651..4421451

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3148_RS21415 (E3148_21590)	gspH	4410807..4411322 (+)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-
E3148_RS21420 (E3148_21595)	gspI	4411319..4411684 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
E3148_RS21425 (E3148_21600)	gspJ	4411681..4412277 (+)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
E3148_RS21430 (E3148_21605)	gspK	4412274..4413260 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
E3148_RS21435 (E3148_21610)	gspL	4413247..4414440 (+)	1194	WP_004194919.1	type II secretion system protein GspL	-
E3148_RS21440 (E3148_21615)	-	4414433..4414918 (+)	486	WP_004145896.1	type II secretion system protein M	-
E3148_RS21445 (E3148_21620)	-	4414908..4415654 (+)	747	WP_004178605.1	type II secretion system protein N	-
E3148_RS21450 (E3148_21625)	pilD	4415651..4416451 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
E3148_RS21455 (E3148_21630)	hrpB	4416499..4418928 (-)	2430	WP_004177388.1	ATP-dependent helicase HrpB	-
E3148_RS21460 (E3148_21635)	thpR	4419001..4419537 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
E3148_RS21465 (E3148_21640)	sfsA	4419537..4420253 (+)	717	WP_020324420.1	DNA/RNA nuclease SfsA	-
E3148_RS21470 (E3148_21645)	dksA	4420416..4420871 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=366151 E3148_RS21450 WP_004145898.1 4415651..4416451(+) (pilD) [Klebsiella pneumoniae strain 2019010306]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=366151 E3148_RS21450 WP_004145898.1 4415651..4416451(+) (pilD) [Klebsiella pneumoniae strain 2019010306]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCATGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425