Detailed information NTG0363869

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FEF28_RS21705
• Genome accession: NZ_CP040391
• Coordinates: 4453801..4454601 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain LSH-KPN25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4448801..4459601

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FEF28_RS21670	gspH	4448957..4449472 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
FEF28_RS21675	gspI	4449469..4449834 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FEF28_RS21680	gspJ	4449831..4450427 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
FEF28_RS21685	gspK	4450424..4451410 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
FEF28_RS21690	gspL	4451397..4452590 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
FEF28_RS21695	-	4452583..4453068 (+)	486	WP_004151943.1	type II secretion system protein M	-
FEF28_RS21700	-	4453058..4453804 (+)	747	WP_004145897.1	type II secretion system protein N	-
FEF28_RS21705	pilD	4453801..4454601 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
FEF28_RS21710	hrpB	4454649..4457078 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
FEF28_RS21715	thpR	4457151..4457687 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
FEF28_RS21720	sfsA	4457687..4458403 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FEF28_RS21725	dksA	4458566..4459021 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=363869 FEF28_RS21705 WP_002888947.1 4453801..4454601(+) (pilD) [Klebsiella pneumoniae strain LSH-KPN25]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=363869 FEF28_RS21705 WP_002888947.1 4453801..4454601(+) (pilD) [Klebsiella pneumoniae strain LSH-KPN25]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425