Detailed information NTG0363643

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FEM43_RS04400
• Genome accession: NZ_CP040363
• Coordinates: 809951..810751 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain R50
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 804951..815751

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FEM43_RS04365 (FEM43_04365)	gspH	805107..805622 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
FEM43_RS04370 (FEM43_04370)	gspI	805619..805984 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FEM43_RS04375 (FEM43_04375)	gspJ	805981..806577 (+)	597	WP_023279276.1	type II secretion system minor pseudopilin GspJ	-
FEM43_RS04380 (FEM43_04380)	gspK	806574..807560 (+)	987	WP_023279277.1	type II secretion system minor pseudopilin GspK	-
FEM43_RS04385 (FEM43_04385)	gspL	807547..808740 (+)	1194	WP_021441216.1	type II secretion system protein GspL	-
FEM43_RS04390 (FEM43_04390)	-	808733..809218 (+)	486	WP_004151943.1	type II secretion system protein M	-
FEM43_RS04395 (FEM43_04395)	-	809208..809954 (+)	747	WP_117074256.1	type II secretion system protein N	-
FEM43_RS04400 (FEM43_04400)	pilD	809951..810751 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
FEM43_RS04405 (FEM43_04405)	hrpB	810799..813228 (-)	2430	WP_117074255.1	ATP-dependent helicase HrpB	-
FEM43_RS04410 (FEM43_04410)	thpR	813301..813837 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
FEM43_RS04415 (FEM43_04415)	sfsA	813837..814553 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FEM43_RS04420 (FEM43_04420)	dksA	814716..815171 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=363643 FEM43_RS04400 WP_004145898.1 809951..810751(+) (pilD) [Klebsiella pneumoniae strain R50]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=363643 FEM43_RS04400 WP_004145898.1 809951..810751(+) (pilD) [Klebsiella pneumoniae strain R50]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425