Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   FE254_RS05260 Genome accession   NZ_CP040349
Coordinates   1189774..1191366 (-) Length   530 a.a.
NCBI ID   WP_090427103.1    Uniprot ID   A0A1H0MHU6
Organism   Pseudomonas guguanensis strain HMFL31     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1184774..1196366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE254_RS05235 (FE254_05290) pilV 1185456..1186037 (-) 582 WP_286586458.1 type IV pilus modification protein PilV -
  FE254_RS05240 (FE254_05295) - 1186046..1186582 (-) 537 WP_279534467.1 GspH/FimT family pseudopilin -
  FE254_RS05245 (FE254_05300) - 1186676..1187170 (-) 495 WP_279534468.1 GspH/FimT family pseudopilin -
  FE254_RS05250 (FE254_05305) thiO 1187229..1188341 (+) 1113 WP_286587181.1 glycine oxidase ThiO -
  FE254_RS05255 (FE254_05310) pilR 1188431..1189771 (-) 1341 WP_286586462.1 sigma-54 dependent transcriptional regulator Regulator
  FE254_RS05260 (FE254_05315) pilS 1189774..1191366 (-) 1593 WP_090427103.1 ATP-binding protein Regulator
  FE254_RS05265 (FE254_05320) - 1191356..1191592 (-) 237 WP_286586463.1 PP0621 family protein -
  FE254_RS05270 (FE254_05325) - 1191780..1192772 (-) 993 WP_090427097.1 outer membrane protein assembly factor BamD -
  FE254_RS05275 (FE254_05330) rluD 1192911..1193885 (+) 975 WP_286586465.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  FE254_RS05280 (FE254_05335) pgeF 1193882..1194613 (+) 732 WP_286586466.1 peptidoglycan editing factor PgeF -
  FE254_RS05285 (FE254_05340) - 1194746..1195336 (+) 591 WP_286586468.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59045.21 Da        Isoelectric Point: 8.1909

>NTDB_id=363549 FE254_RS05260 WP_090427103.1 1189774..1191366(-) (pilS) [Pseudomonas guguanensis strain HMFL31]
MTADLSVQGSTQGRRILRLYHFYRLSIGLALILMISSNLDEQLLDVVHIDLFRTGSWVYLILNIFIAVLVQKPKHLMQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVIAVAIANILLRGRIGLLIAAIAAMALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLQVSESLAEQRAADVANLEELNEQILQRMRTGILVLDTQHRVLLANQGATQLLGRGELTGKIID
PHCPELVKRLQQWLHNPTLRPDSLQAQADGPMLQPSFISLQRGEQQHTLVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPAD
RQIHLETQGGSLQTRMDPHQLTQVLTNLVQNGLRYSAQKHRLGQVWLRLFRDEVSDLPVLEVLDDGPGVPPEQEQHIFEP
FYTTENKGTGLGLYISRELCQGNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=363549 FE254_RS05260 WP_090427103.1 1189774..1191366(-) (pilS) [Pseudomonas guguanensis strain HMFL31]
GTGACCGCTGATCTCAGCGTGCAGGGCAGCACCCAGGGGCGACGCATCCTCAGGCTCTATCATTTTTATCGCCTGAGCAT
CGGCCTGGCGCTGATCCTGATGATTTCCAGCAACCTCGACGAGCAGCTGCTGGATGTCGTGCATATCGACCTGTTTCGAA
CAGGCAGCTGGGTCTACCTGATCCTCAACATCTTCATCGCCGTGCTGGTGCAAAAGCCCAAGCACCTGATGCAGCTGTTC
AGCCTGGCGCTGGTGGACGTGATCTTCCTCTCCGCGCTGTTCTACGCCGCCGGCGGCACACCCAGCGGCATCGGCAACCT
GCTGGTGATCGCCGTAGCCATCGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGCTGATCGCCGCCATCGCCGCCATGG
CGCTGATCTACCTGACCTTCTACCTCAGCCTCAGCCGCCCGGCCGCTGCCGCCCAGTACGTGCAGGCCGGCGCCCTTGGC
GCACTGTGCTTCGCCGGTGCGCTGTTCATCCAGGGCATCACGCGCCGCCTGCAGGTGAGTGAGAGCCTGGCCGAACAGCG
CGCAGCCGATGTGGCCAATCTCGAAGAGCTCAACGAGCAGATCCTGCAGCGCATGCGCACCGGCATCCTGGTACTCGATA
CCCAGCACCGCGTGCTGCTGGCCAACCAGGGCGCCACCCAGTTGCTGGGCCGCGGCGAACTGACCGGCAAGATCATCGAC
CCGCACTGCCCCGAGCTGGTCAAACGCCTGCAGCAGTGGCTGCACAACCCGACCCTGCGCCCGGACAGCCTGCAGGCGCA
GGCCGACGGTCCGATGCTGCAGCCCAGCTTCATCTCCCTGCAACGCGGCGAGCAGCAGCACACCCTGGTATTTCTCGACG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAACTCAAGCTCGCCTCCCTCGGCCGCCTGACTGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTCGGCGCCATCAGCCACGCCGCCCAGCTGCTGCAGGAATCGGAAGACCTGCAAGGCCCGGATCAACG
CCTTGCGCAGATCATCCAGGACCACTCCAAGCGTATGAACCTGGTGATCGAGAACGTCCTGCAGCTGTCCCGCCGGCGCC
AGGCCGAGCCGCAACTGCTGGATCTGAAATACTGGCTGCACCGTTTCGCCAGCGAGTTCCGCAGCTCGGCCCCGGCTGAC
CGGCAGATCCATCTGGAAACCCAGGGCGGCAGCCTGCAAACACGCATGGACCCGCACCAGCTGACCCAGGTGCTGACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGCGCAGAAGCACCGGCTCGGCCAGGTCTGGCTGCGCCTGTTCCGCGACGAGG
TCAGCGACCTGCCGGTACTGGAAGTGCTCGACGACGGCCCCGGCGTGCCGCCCGAGCAGGAACAGCACATCTTCGAGCCC
TTCTACACCACCGAAAACAAGGGCACCGGCCTCGGCCTGTATATCTCCCGCGAGCTGTGCCAGGGCAACCAGGCCCGCCT
CGACTATAAACCTCGTGAAGGGGGCGGCAGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

PAS domain (PAS_8)

(199-254)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H0MHU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

66.667

97.925

0.653

Multiple sequence alignment