Detailed information NTG0036316

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SMI_RS04825
• Genome accession: NC_013853
• Coordinates: 908458..910698 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942437.1
• Uniprot ID: D3H8K8
• Organism: Streptococcus mitis B6
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 903458..915698

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMI_RS04790 (smi_0949)	pyrH	903592..904329 (+)	738	WP_000002996.1	UMP kinase	-
SMI_RS04795 (smi_0950)	frr	904338..904895 (+)	558	WP_001262225.1	ribosome recycling factor	-
SMI_RS04800 (smi_0951)	cvfB	904955..905809 (+)	855	WP_001095453.1	RNA-binding virulence regulatory protein CvfB	-
SMI_RS04805 (smi_0952)	-	905818..906033 (+)	216	WP_001232084.1	YozE family protein	-
SMI_RS04810 (smi_0953)	-	906118..907122 (+)	1005	WP_000658206.1	PhoH family protein	-
SMI_RS04815 (smi_0954)	-	907209..907778 (+)	570	WP_000443760.1	GNAT family N-acetyltransferase	-
SMI_RS04820 (smi_0955)	comEA/celA/cilE	907845..908474 (+)	630	WP_000387350.1	helix-hairpin-helix domain-containing protein	Machinery gene
SMI_RS04825 (smi_0956)	comEC/celB	908458..910698 (+)	2241	WP_000942437.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SMI_RS10765	-	910840..911064 (+)	225	WP_231840218.1	hypothetical protein	-
SMI_RS04830 (smi_0957)	-	911097..911684 (+)	588	WP_000939892.1	ATP-binding cassette domain-containing protein	-
SMI_RS04835 (smi_0958)	-	911688..912872 (+)	1185	WP_000655831.1	hypothetical protein	-
SMI_RS04840 (smi_0959)	infC	913180..913710 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
SMI_RS04845 (smi_0960)	rpmI	913743..913943 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
SMI_RS04850 (smi_0961)	rplT	913995..914354 (+)	360	WP_000124834.1	50S ribosomal protein L20	-
SMI_RS04855 (smi_0962)	-	914413..914793 (+)	381	WP_000157153.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84512.93 Da
• Isoelectric Point: 9.5653

>NTDB_id=36316 SMI_RS04825 WP_000942437.1 908458..910698(+) (comEC/celB) [Streptococcus mitis B6]
MLQWIKNIPLPLIYLSFLLLWFYYAIFSASYLALLGFVFLLVCLFIQFPGKSAGKVLVICGIFGFWFLFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKADGRIFQVYYKLQSEEGKETFQALTDLHEIGLEGKLSEPEGKRNFGGFDYQAY
LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFII
MPNFFLTTGGVLSCAYAFILTMTSKEGEGFKAIARESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDMVLLPLLSI
LFALSFLYPVIQLNFIFEWLEGIIRLVSQVASRSLVFGQPNAWLLILLLISLALVYDLRKNIKRLAVLSLLITGLFFLTK
YPLENEITMLDVGQGESIFLRDVTGKTILIDVAGKAEYNKKIEKWQEKTTTSNAQRTLIPYLKSRGVAKIDQLILTNMDK
EHVGDLLEVTKVFHVGEILVSKGSLKQKEFVTELQATQTKVRSVTAGENLPIFGSQLEVLSPRKIGDGGHEDSLVLYGKL
LDKNFLFTGNLEEKGEKDLLKQYPDLEVDVLKAGQHGSKNSSSSAFLEKLKPDFTLISVGKNNRTKLPHQETLTRLETIN
SKVYRTDQQGAIRFKGWKNWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=36316 SMI_RS04825 WP_000942437.1 908458..910698(+) (comEC/celB) [Streptococcus mitis B6]
ATGTTACAGTGGATTAAGAATATCCCCCTTCCCCTAATTTACCTGAGTTTTTTGTTACTTTGGTTTTATTACGCTATTTT
CTCAGCATCCTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTTTGTCTCTTTATTCAATTTCCTGGGAAATCAGCAG
GCAAAGTTCTAGTGATTTGTGGAATCTTTGGATTTTGGTTTCTGTTTCAAAATTGGCAACAGAGTCAAGCAAGTCAAAAT
TTGGCGGATTCTGTTGAAAGGGTACGGATTCTGCCCGACACTATTAAGGTCAATGGTGATAGTCTGTCCTTTCGTGGCAA
GGCTGATGGACGCATTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGGGAAAGAAACCTTTCAAGCCTTAACAGACC
TTCATGAGATAGGACTAGAAGGGAAGCTTTCTGAGCCAGAAGGGAAGAGAAATTTTGGTGGCTTTGACTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAAGAATCCAGTCACTTCAAAAGGTTGGCAGTTGGGATATAGG
AGAAAATCTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACACATTTTCCAGACCCTATGCGCAATTACATGA
CAGGGCTTTTGCTAGGACATCTGGACACAGATTTTGAGGAGATGAATGAGCTTTATTCTAGTTTAGGAATTATCCACCTT
TTTGCCTTGTCGGGTATGCAGGTAGGCTTTTTCATGGATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACCTATCCCTTTTCCCTTATTTATGCAGGTCTGACAGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTACAAAAGTTACTGGCTCAACATGGTGTTAAGGGCTTGGATAATTTTGCCTTGACTGTGCTTGTCCTCTTTATTATC
ATGCCCAACTTTTTCCTGACTACAGGAGGAGTTTTGTCATGTGCTTATGCTTTTATCTTGACTATGACTAGCAAAGAAGG
GGAGGGGTTTAAGGCTATTGCTAGAGAAAGTCTAGTCATTTCCTTAGGAATATTGCCCATTCTATCCTTCTATTTTGCAG
AATTTCAACCTTGGTCCATCCTCTTGACCTTTGTCTTTTCCTTTCTATTTGACATGGTCCTCTTACCGCTCTTGTCTATC
TTATTTGCCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAATTTTATCTTTGAATGGTTGGAAGGGATTATTCGCTTGGT
ATCACAGGTGGCAAGTAGATCGCTTGTCTTTGGACAACCTAATGCGTGGCTTTTAATTTTATTGTTAATTTCCTTGGCTT
TAGTCTATGATTTGAGAAAAAACATTAAAAGGCTAGCAGTGTTGAGCTTATTGATTACAGGTCTCTTTTTCCTGACCAAG
TATCCACTGGAAAATGAAATTACCATGCTGGATGTGGGGCAAGGCGAAAGTATTTTCCTACGGGATGTAACTGGTAAAAC
CATTCTCATAGATGTCGCTGGCAAGGCAGAATATAATAAGAAAATCGAAAAATGGCAAGAAAAGACGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCCAAGATTGACCAGCTGATTTTGACAAATATGGACAAG
GAACATGTTGGAGATTTGTTGGAGGTGACCAAGGTTTTTCATGTCGGAGAGATTTTAGTGTCCAAAGGCAGTTTGAAACA
GAAGGAATTTGTGACAGAACTACAGGCAACTCAAACCAAGGTGCGCAGTGTGACAGCAGGAGAGAACTTGCCAATTTTTG
GAAGTCAGTTAGAAGTCTTATCTCCAAGGAAAATTGGAGATGGAGGTCATGAAGATTCTCTAGTTCTGTATGGGAAACTC
TTGGATAAAAACTTTCTTTTCACAGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGTTGAAGCAGTATCCTGACCTAGA
AGTGGATGTTTTGAAAGCTGGTCAACATGGCTCTAAAAACTCATCAAGTTCAGCCTTTCTAGAAAAACTCAAACCAGATT
TTACTCTCATCTCAGTTGGAAAGAACAATCGAACGAAACTCCCCCATCAGGAAACCTTGACACGACTGGAAACTATCAAT
AGCAAAGTTTACCGAACTGACCAGCAAGGAGCTATACGCTTTAAAGGTTGGAAAAATTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-698)

• Competence protein (Competence) (216-458)

• Domain of unknown function (DUF4131) (DUF4131) (31-176)

3D structure

• AlphaFold DB: D3H8K8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	93.164	100	0.932
comEC/celB	Streptococcus mitis NCTC 12261	92.617	99.866	0.925
comEC/celB	Streptococcus pneumoniae TIGR4	91.287	100	0.913
comEC/celB	Streptococcus pneumoniae Rx1	90.751	100	0.908
comEC/celB	Streptococcus pneumoniae D39	90.751	100	0.908
comEC/celB	Streptococcus pneumoniae R6	90.751	100	0.908
comEC	Lactococcus lactis subsp. cremoris KW2	44.355	99.732	0.442