Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SMI_RS01665 Genome accession   NC_013853
Coordinates   313568..314398 (+) Length   276 a.a.
NCBI ID   WP_001036774.1    Uniprot ID   D3H6U9
Organism   Streptococcus mitis B6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 308568..319398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMI_RS01630 (smi_0313) asnA 308659..309651 (+) 993 WP_000747996.1 aspartate--ammonia ligase -
  SMI_RS01635 (smi_0314) rsmD 309716..310255 (+) 540 WP_000706956.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SMI_RS01640 (smi_0315) coaD 310245..310733 (+) 489 WP_001280758.1 pantetheine-phosphate adenylyltransferase -
  SMI_RS01645 (smi_0316) sepM 310717..311754 (+) 1038 WP_012972442.1 SepM family pheromone-processing serine protease Regulator
  SMI_RS01650 - 311812..312042 (+) 231 WP_000250385.1 DUF1146 family protein -
  SMI_RS01655 (smi_0317) murA 312065..313348 (+) 1284 WP_000411895.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SMI_RS01660 (smi_0318) - 313341..313529 (+) 189 WP_164925506.1 DNA-directed RNA polymerase subunit beta -
  SMI_RS01665 (smi_0319) endA 313568..314398 (+) 831 WP_001036774.1 DNA-entry nuclease EndA Machinery gene
  SMI_RS01670 (smi_0320) - 314660..315991 (+) 1332 WP_000390035.1 hemolysin family protein -
  SMI_RS01680 (smi_0322) amiA 316528..318507 (+) 1980 WP_000749702.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 276 a.a.        Molecular weight: 30131.79 Da        Isoelectric Point: 9.9432

>NTDB_id=36296 SMI_RS01665 WP_001036774.1 313568..314398(+) (endA) [Streptococcus mitis B6]
MNKKTRQTLIGLLVLLLLSAGSYYIKQMQSTANSPKTKVSQKKQASEAPSQELAESVLTDAVKGQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKALDKNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTHTQ

Nucleotide


Download         Length: 831 bp        

>NTDB_id=36296 SMI_RS01665 WP_001036774.1 313568..314398(+) (endA) [Streptococcus mitis B6]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTATCTGCAGGGAGCTACTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACTAAGGTTAGTCAGAAAAAACAAGCTTCTGAAGCTCCTAGTCAAGAATTGG
CAGAAAGTGTCTTAACAGACGCAGTCAAAGGCCAAATAAAAGGGAGTCTTGAATGGAATGGCTCAGGTGCTTTTATTGTC
AATGGTAATAAAACAAACCTAGATGCCAAAGTTTCAAGTAAGCCCTACGCTGATAATAAAACAAAGACAGTGGGCAAGGA
AACCGTGCCAACTGTAGCAAATGCTCTCTTGTCTAAGGCAACTCGCCAGTACAAGAATCGTGAAGAAACGGGGAATGGTT
CGACTTCATGGACTCCTCCAGGCTGGCATCAGGTTAAGAATCTAAAGGGTACTTATACCCATGCGGTCGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAAAC
AGCCTGGGCAAATCAGGCACAAGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGCATGGTACGTAAGGCCTTGGATA
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCGAATGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGATGGAGAATTGGAATTTAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCATACTCAATGA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3H6U9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

94.891

99.275

0.942
  endA Streptococcus pneumoniae D39

94.891

99.275

0.942
  endA Streptococcus pneumoniae R6

94.891

99.275

0.942
  endA Streptococcus pneumoniae TIGR4

94.891

99.275

0.942

Multiple sequence alignment