Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FEF05_RS03230 Genome accession   NZ_CP040243
Coordinates   613732..614313 (-) Length   193 a.a.
NCBI ID   WP_010786056.1    Uniprot ID   A0A859IE21
Organism   Glaesserella parasuis strain HPS-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 608732..619313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEF05_RS03190 (FEF05_03195) - 609079..609717 (+) 639 WP_149350715.1 nitroreductase family protein -
  FEF05_RS03195 (FEF05_03200) - 609806..610339 (+) 534 WP_015940082.1 TIGR00645 family protein -
  FEF05_RS03200 (FEF05_03205) - 610424..611722 (-) 1299 WP_075592116.1 NAD(P)/FAD-dependent oxidoreductase -
  FEF05_RS03220 (FEF05_03225) greB 612256..612735 (-) 480 WP_010786058.1 transcription elongation factor GreB -
  FEF05_RS03225 (FEF05_03230) - 612735..613670 (-) 936 WP_149350716.1 KpsF/GutQ family sugar-phosphate isomerase -
  FEF05_RS03230 (FEF05_03235) pilF 613732..614313 (-) 582 WP_010786056.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FEF05_RS03235 (FEF05_03240) - 614553..615524 (+) 972 WP_016528180.1 IS110 family transposase -
  FEF05_RS03240 (FEF05_03245) - 615568..616728 (-) 1161 WP_035492316.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FEF05_RS03245 (FEF05_03250) - 616929..617156 (-) 228 WP_016528332.1 hypothetical protein -
  FEF05_RS03250 (FEF05_03255) - 617262..617498 (-) 237 WP_015940086.1 hypothetical protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.29 Da        Isoelectric Point: 8.4221

>NTDB_id=362831 FEF05_RS03230 WP_010786056.1 613732..614313(-) (pilF) [Glaesserella parasuis strain HPS-1]
MTFAKFFRNLTACSVALWLVACTNQTAKVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=362831 FEF05_RS03230 WP_010786056.1 613732..614313(-) (pilF) [Glaesserella parasuis strain HPS-1]
ATGACCTTCGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCGTTATGGCTTGTTGCTTGTACAAATCAAACCGC
AAAGGTGGATTTTAACCGTTCAGAAGCCGTCAAAGCTCGTATTAATTTGGCTCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACATGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A859IE21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

97.927

100

0.979
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.251

92.746

0.42

Multiple sequence alignment