Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SMI_RS00135 Genome accession   NC_013853
Coordinates   25663..27024 (+) Length   453 a.a.
NCBI ID   WP_164925536.1    Uniprot ID   -
Organism   Streptococcus mitis B6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20663..32024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMI_RS00105 (smi_0021) comW 22057..22293 (+) 237 WP_000941921.1 sigma(X)-activator ComW Regulator
  SMI_RS00110 (smi_0022) - 22524..23810 (+) 1287 WP_012972411.1 adenylosuccinate synthase -
  SMI_RS00115 (smi_0024) tadA 24011..24478 (+) 468 WP_000386764.1 tRNA adenosine(34) deaminase TadA -
  SMI_RS00125 (smi_0026) - 24665..25108 (+) 444 WP_000701987.1 dUTP diphosphatase -
  SMI_RS00130 (smi_0027) - 25110..25649 (+) 540 WP_000691251.1 histidine phosphatase family protein -
  SMI_RS00135 (smi_0028) radA 25663..27024 (+) 1362 WP_164925536.1 DNA repair protein RadA Machinery gene
  SMI_RS00140 (smi_0029) - 27096..27791 (+) 696 WP_000672724.1 TIGR00266 family protein -
  SMI_RS00145 (smi_0030) - 27951..28448 (+) 498 WP_000119799.1 beta-class carbonic anhydrase -
  SMI_RS00150 (smi_0031) - 28473..29288 (+) 816 WP_000828208.1 PrsW family intramembrane metalloprotease -
  SMI_RS00155 (smi_0032) - 29584..30750 (-) 1167 WP_000163002.1 IS30-like element ISSmi1 family transposase -
  SMI_RS00160 (smi_0033) - 30990..31958 (+) 969 WP_000010168.1 ribose-phosphate diphosphokinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.91 Da        Isoelectric Point: 6.5137

>NTDB_id=36280 SMI_RS00135 WP_164925536.1 25663..27024(+) (radA) [Streptococcus mitis B6]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRVEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKAFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=36280 SMI_RS00135 WP_164925536.1 25663..27024(+) (radA) [Streptococcus mitis B6]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTA
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGAAAATCAACCCTTCTCTTACA
AGTCTCAACCCAGTTGTCTCAAGTGGGGACTGTTCTCTACGTCAGTGGGGAGGAGTCTGCTCAGCAGATTAAACTACGTG
CAGAGCGTTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGCGTAGAGGTAGAG
AGAATCCAGCCAGACTTTCTCATTATTGACTCAATTCAAACCATTATGTCTCCTGAGATTTCAGGGGTACAGGGATCTGT
TTCTCAAGTGCGTGAGGTAACGGCTGAACTTATGCAGCTGGCCAAAACCAATAACATTGCCATCTTTATCGTAGGGCATG
TGACCAAGGAAGGAACCTTGGCCGGTCCTCGTATGTTGGAGCACATGGTAGATACGGTACTTTACTTTGAAGGGGAGCGT
CACCATACCTTCCGTATTCTGAGAGCGGTCAAAAACCGTTTTGGTTCAACAAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGTTTGGTTGAGGTCCTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCGTCAATCG
TTGTGACTATGGAAGGGACGCGTCCAATTTTAGCAGAGGTTCAGGCTTTGGTGACACCGACCATGTTTGGGAACGCCAAG
CGCACGACGACAGGACTTGATTTTAATCGTGCGAGTCTGATTATGGCTGTTTTAGAAAAGCGTGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTCAAATTGGATGAACCTGCCATTGACTTAGCCGTCGCTGTTGCTA
TTGCTTCTAGCTATAAAGACAAGCCAACCAACCCTCAGGAATGTTTTGTAGGAGAACTAGGTTTGACTGGAGAAATTCGG
CGCGTGAATCGTATCGAGCAACGCATCAATGAAGCTGCTAAACTGGGTTTTACTAAGATTTATGTACCTAAGAATTCCTT
AACAGGAATCACTCCACCCAAGGAAATTCAGGTCATTGGGGTGACAACGATTCAGGAAGTTTTGAAAAAAGCATTCTCCT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA Streptococcus pneumoniae Rx1

99.117

100

0.991
  radA Streptococcus pneumoniae D39

99.117

100

0.991
  radA Streptococcus pneumoniae R6

99.117

100

0.991
  radA Streptococcus pneumoniae TIGR4

99.117

100

0.991
  radA Bacillus subtilis subsp. subtilis str. 168

62.719

100

0.631

Multiple sequence alignment