Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FAY22_RS20880 Genome accession   NZ_CP040128
Coordinates   4518206..4519687 (-) Length   493 a.a.
NCBI ID   WP_146332722.1    Uniprot ID   A0A5B8NAU3
Organism   Noviherbaspirillum sp. UKPF54     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4513206..4524687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAY22_RS20860 (FAY22_20860) aroKB 4513731..4515369 (+) 1639 Protein_4140 bifunctional shikimate kinase/3-dehydroquinate synthase AroKB -
  FAY22_RS20865 (FAY22_20865) - 4515366..4516499 (+) 1134 WP_146332716.1 deoxyguanosinetriphosphate triphosphohydrolase -
  FAY22_RS20870 (FAY22_20870) - 4516507..4517595 (-) 1089 WP_146332718.1 enoyl-CoA hydratase/isomerase family protein -
  FAY22_RS20875 (FAY22_20875) hpf 4517741..4518088 (-) 348 WP_146332720.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  FAY22_RS20880 (FAY22_20880) rpoN/rpoN1 4518206..4519687 (-) 1482 WP_146332722.1 RNA polymerase factor sigma-54 Machinery gene
  FAY22_RS20885 (FAY22_20885) lptB 4519706..4520446 (-) 741 WP_146332724.1 LPS export ABC transporter ATP-binding protein -
  FAY22_RS20890 (FAY22_20890) lptA 4520448..4520996 (-) 549 WP_146332726.1 lipopolysaccharide transport periplasmic protein LptA -
  FAY22_RS20895 (FAY22_20895) lptC 4521030..4521617 (-) 588 WP_146332728.1 LPS export ABC transporter periplasmic protein LptC -
  FAY22_RS20900 (FAY22_20900) - 4521614..4522138 (-) 525 WP_146332731.1 HAD family hydrolase -
  FAY22_RS20905 (FAY22_20905) - 4522138..4523166 (-) 1029 WP_146332733.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54726.24 Da        Isoelectric Point: 5.3874

>NTDB_id=362406 FAY22_RS20880 WP_146332722.1 4518206..4519687(-) (rpoN/rpoN1) [Noviherbaspirillum sp. UKPF54]
MKQTLQLRVSQHLALTPQLQQSIRLLQLSTLELHQELEQILSDNPMLERVDDPLDHSVRLLADGAITPGNGSADTAAPEA
SAPAASDEGEGGFDSPPEGGEAASSAEGEWSFDDVARTSKAPEDDDARPQLEAHETTLREHLLEQMRVTVHSQRDRALVE
LIIDALDDNGYLEEPLEEIHARLPAELEIELEELQVALKLVQSFEPAGVGARNASECLAIQIARFPKVPLVTRRRALEIV
ENHLALFAQRDFNKLKKALDCDDEDLREAHAVIRRCNPHPGAMFASDASDYVVPDVIVKKAKNGWQVMLNHDVMPRLRVN
ALYANILKQSKGEGSLTAQLQEAKWLIKNMRQRFDTILRVAQAIVDRQRNFFSHGAVAMRPLVLREIADTLGLHESTISR
VTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIKQLIGAEDPKNPFSDSKIADMLGEQGMVVARRTVAKYRE
ALKIPPVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=362406 FAY22_RS20880 WP_146332722.1 4518206..4519687(-) (rpoN/rpoN1) [Noviherbaspirillum sp. UKPF54]
ATGAAACAGACTCTCCAACTTCGCGTCTCGCAACATCTGGCGCTCACGCCGCAGTTGCAGCAATCGATCCGGTTACTGCA
GCTATCCACGCTCGAGCTGCATCAGGAGCTGGAGCAGATCCTGTCCGACAATCCGATGCTGGAACGGGTCGACGACCCGC
TCGATCATTCGGTGCGCCTGCTGGCCGACGGCGCCATCACGCCCGGCAACGGCAGCGCCGACACCGCGGCACCGGAAGCG
AGCGCGCCGGCGGCCTCGGACGAAGGCGAAGGCGGATTCGATAGCCCGCCCGAAGGCGGTGAAGCAGCGTCGTCGGCCGA
GGGCGAATGGAGCTTCGACGACGTTGCGCGCACCTCGAAGGCGCCGGAGGACGACGATGCGCGCCCGCAGCTGGAAGCGC
ACGAAACCACCCTGCGCGAACACCTGCTGGAACAGATGCGCGTGACCGTGCACAGCCAGCGCGACCGCGCACTGGTCGAA
CTGATCATCGACGCGCTGGACGACAACGGCTACCTGGAAGAGCCGCTGGAGGAAATCCATGCTCGCCTGCCGGCCGAGCT
GGAAATCGAACTGGAAGAGCTGCAGGTCGCACTGAAGCTGGTGCAAAGCTTCGAACCGGCCGGCGTCGGGGCGCGCAATG
CGTCCGAGTGCCTGGCGATCCAGATCGCACGCTTTCCGAAAGTGCCGCTGGTGACGCGCAGGAGAGCGCTGGAGATAGTC
GAAAACCATCTCGCGCTGTTTGCGCAACGCGACTTCAACAAGCTCAAGAAGGCGCTCGATTGCGACGACGAAGACTTGCG
TGAAGCGCATGCGGTGATCCGCCGCTGCAATCCGCATCCCGGCGCCATGTTCGCGTCCGATGCATCGGACTATGTCGTGC
CGGATGTAATTGTCAAAAAAGCAAAGAACGGCTGGCAAGTGATGCTCAATCACGATGTCATGCCCCGCCTGCGCGTCAAT
GCGCTGTACGCGAATATCCTCAAGCAGAGCAAGGGTGAGGGTTCACTGACCGCGCAGCTGCAGGAAGCCAAGTGGCTGAT
CAAGAACATGCGGCAGCGCTTCGACACGATCTTGCGCGTTGCACAAGCGATTGTTGATCGGCAAAGAAATTTCTTCTCGC
ATGGCGCAGTCGCGATGCGCCCCCTTGTCTTGCGTGAAATAGCTGATACACTGGGTTTACACGAAAGTACTATCTCACGT
GTAACAACTCAAAAATATATGCTGACCCCGCATGGCATGTTTGAGTTGAAGTACTTCTTCGGTAGCCACGTCGCGACCGA
AGCCGGGGGCGAAGCTTCGTCGACCGCGATACGGGCACTGATCAAACAATTGATAGGAGCTGAAGACCCCAAGAACCCAT
TCTCTGATAGCAAAATAGCGGATATGTTGGGTGAACAGGGCATGGTCGTTGCGCGTCGCACAGTCGCGAAGTACCGCGAA
GCTCTGAAAATCCCTCCAGTCAATCTGCGCAAATCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8NAU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.516

100

0.619

Multiple sequence alignment