Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   FDM99_RS01165 Genome accession   NZ_CP040080
Coordinates   222237..222977 (-) Length   246 a.a.
NCBI ID   WP_032025038.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain SP304     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217237..227977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDM99_RS01145 (FDM99_01145) aroB 217779..218861 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  FDM99_RS01150 (FDM99_01150) aroK 218890..219459 (-) 570 WP_000643712.1 shikimate kinase AroK -
  FDM99_RS01155 (FDM99_01155) pilQ 219471..221636 (-) 2166 Protein_217 type IV pilus secretin PilQ -
  FDM99_RS01160 (FDM99_01160) pilP 221699..222226 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  FDM99_RS01165 (FDM99_01165) pilO 222237..222977 (-) 741 WP_032025038.1 type 4a pilus biogenesis protein PilO Machinery gene
  FDM99_RS01170 (FDM99_01170) pilN 222974..223615 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  FDM99_RS01175 (FDM99_01175) pilM 223615..224673 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  FDM99_RS01180 (FDM99_01180) ponA 224835..227390 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27847.64 Da        Isoelectric Point: 4.8357

>NTDB_id=361905 FDM99_RS01165 WP_032025038.1 222237..222977(-) (pilO) [Acinetobacter baumannii strain SP304]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNVQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=361905 FDM99_RS01165 WP_032025038.1 222237..222977(-) (pilO) [Acinetobacter baumannii strain SP304]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGTTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

68.724

98.78

0.679

Multiple sequence alignment