Detailed information NTG0361528

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EA674_RS14640
• Genome accession: NZ_CP039993
• Coordinates: 3016216..3017334 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain TG22182
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3011216..3022334

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EA674_RS14630 (EA674_014630)	-	3014332..3015024 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
EA674_RS14635 (EA674_014635)	pilT	3015151..3016188 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EA674_RS14640 (EA674_014640)	pilU	3016216..3017334 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EA674_RS14645 (EA674_014645)	fur	3017404..3017841 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EA674_RS14650 (EA674_014650)	-	3017953..3018351 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EA674_RS14655 (EA674_014655)	-	3018406..3018729 (-)	324	WP_000442587.1	RnfH family protein	-
EA674_RS14660 (EA674_014660)	-	3018729..3019796 (-)	1068	WP_002019572.1	hypothetical protein	-
EA674_RS14670 (EA674_014670)	-	3019949..3020404 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EA674_RS14675 (EA674_014675)	-	3020657..3021505 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=361528 EA674_RS14640 WP_000347039.1 3016216..3017334(+) (pilU) [Acinetobacter baumannii strain TG22182]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=361528 EA674_RS14640 WP_000347039.1 3016216..3017334(+) (pilU) [Acinetobacter baumannii strain TG22182]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395