Detailed information NTG0361294

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FC646_RS05480
• Genome accession: NZ_CP039951
• Coordinates: 1147536..1148336 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 18KM2445b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1142536..1153336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FC646_RS05445 (FC646_05530)	gspH	1142692..1143207 (+)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-
FC646_RS05450 (FC646_05535)	gspI	1143204..1143569 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FC646_RS05455 (FC646_05540)	gspJ	1143566..1144162 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
FC646_RS05460 (FC646_05545)	gspK	1144159..1145145 (+)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
FC646_RS05465 (FC646_05550)	gspL	1145132..1146325 (+)	1194	WP_032434208.1	type II secretion system protein GspL	-
FC646_RS05470 (FC646_05555)	-	1146318..1146803 (+)	486	WP_004145896.1	type II secretion system protein M	-
FC646_RS05475 (FC646_05560)	-	1146793..1147539 (+)	747	WP_032434210.1	type II secretion system protein N	-
FC646_RS05480 (FC646_05565)	pilD	1147536..1148336 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
FC646_RS05485 (FC646_05570)	hrpB	1148384..1150813 (-)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
FC646_RS05490 (FC646_05575)	thpR	1150886..1151422 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
FC646_RS05495 (FC646_05580)	sfsA	1151422..1152138 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FC646_RS05500 (FC646_05585)	dksA	1152301..1152756 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=361294 FC646_RS05480 WP_004213441.1 1147536..1148336(+) (pilD) [Klebsiella pneumoniae strain 18KM2445b]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=361294 FC646_RS05480 WP_004213441.1 1147536..1148336(+) (pilD) [Klebsiella pneumoniae strain 18KM2445b]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425