Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FCJ76_RS12415 Genome accession   NZ_CP039935
Coordinates   2409280..2410350 (-) Length   356 a.a.
NCBI ID   WP_128738022.1    Uniprot ID   -
Organism   Bacillus subtilis strain H19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2404280..2415350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCJ76_RS12370 tapA 2404758..2405519 (-) 762 WP_101169542.1 amyloid fiber anchoring/assembly protein TapA -
  FCJ76_RS12375 yqzG 2405792..2406118 (+) 327 WP_024573388.1 YqzG/YhdC family protein -
  FCJ76_RS12380 spoIITA 2406160..2406339 (-) 180 WP_014480252.1 YqzE family protein -
  FCJ76_RS12385 comGG 2406410..2406784 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  FCJ76_RS12390 comGF 2406785..2407168 (-) 384 WP_128738019.1 ComG operon protein ComGF Machinery gene
  FCJ76_RS12395 comGE 2407194..2407541 (-) 348 WP_128738020.1 ComG operon protein 5 Machinery gene
  FCJ76_RS12400 comGD 2407525..2407956 (-) 432 WP_024573390.1 comG operon protein ComGD Machinery gene
  FCJ76_RS12405 comGC 2407946..2408242 (-) 297 WP_024573391.1 comG operon protein ComGC Machinery gene
  FCJ76_RS12410 comGB 2408256..2409293 (-) 1038 WP_128738021.1 comG operon protein ComGB Machinery gene
  FCJ76_RS12415 comGA 2409280..2410350 (-) 1071 WP_128738022.1 competence protein ComGA Machinery gene
  FCJ76_RS21530 - 2410561..2410686 (-) 126 WP_003230155.1 hypothetical protein -
  FCJ76_RS12425 corA 2410752..2411705 (-) 954 WP_004399136.1 magnesium transporter CorA -
  FCJ76_RS12430 yqhB 2411848..2413176 (+) 1329 WP_003230150.1 hemolysin family protein -
  FCJ76_RS12435 rsbRD 2413229..2414065 (-) 837 WP_015384093.1 RsbT co-antagonist protein RsbRD -
  FCJ76_RS12445 mgsR 2414289..2414669 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  FCJ76_RS12450 yqgY 2414901..2415146 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40444.62 Da        Isoelectric Point: 9.0175

>NTDB_id=361140 FCJ76_RS12415 WP_128738022.1 2409280..2410350(-) (comGA) [Bacillus subtilis strain H19]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAVIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=361140 FCJ76_RS12415 WP_128738022.1 2409280..2410350(-) (comGA) [Bacillus subtilis strain H19]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTGTCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCCACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAATACCAGGAGAGCTAGTGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997

Multiple sequence alignment