Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   GTW26_RS26070 Genome accession   NZ_CP047643
Coordinates   5534150..5534689 (-) Length   179 a.a.
NCBI ID   WP_003141352.1    Uniprot ID   A0A0H2ZFW8
Organism   Pseudomonas aeruginosa CI27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5529150..5539689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTW26_RS26050 (GTW26_26005) - 5529836..5532091 (-) 2256 WP_003134886.1 DUF1631 domain-containing protein -
  GTW26_RS26055 (GTW26_26010) nadC 5532377..5533225 (+) 849 WP_003141348.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GTW26_RS26065 (GTW26_26020) - 5533388..5534086 (-) 699 WP_016254081.1 hypothetical protein -
  GTW26_RS26070 (GTW26_26025) pilE 5534150..5534689 (-) 540 WP_003141352.1 pilin Machinery gene
  GTW26_RS26075 (GTW26_26030) pilB 5534918..5536621 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTW26_RS26080 (GTW26_26035) pilC 5536625..5537842 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  GTW26_RS26085 (GTW26_26040) pilD 5537845..5538717 (+) 873 WP_003141356.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  GTW26_RS26090 (GTW26_26045) coaE 5538714..5539325 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  GTW26_RS26095 (GTW26_26050) yacG 5539322..5539522 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18083.45 Da        Isoelectric Point: 5.0804

>NTDB_id=360453 GTW26_RS26070 WP_003141352.1 5534150..5534689(-) (pilE) [Pseudomonas aeruginosa CI27]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=360453 GTW26_RS26070 WP_003141352.1 5534150..5534689(-) (pilE) [Pseudomonas aeruginosa CI27]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTGCGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZFW8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.243

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363

Multiple sequence alignment