Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GTP13_RS04645 Genome accession   NZ_CP047378
Coordinates   948864..949304 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain CAU16175     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943864..954304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTP13_RS04625 coaE 944354..944974 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  GTP13_RS31100 - 944999..945043 (+) 45 WP_120795372.1 protein YacM -
  GTP13_RS04630 guaC 945199..946242 (+) 1044 WP_001217338.1 GMP reductase -
  GTP13_RS04635 hofC 946277..947479 (-) 1203 WP_000157234.1 protein transport protein HofC -
  GTP13_RS04640 pilB 947469..948854 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  GTP13_RS04645 pilA 948864..949304 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  GTP13_RS04650 nadC 949507..950400 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GTP13_RS04655 ampD 950488..951039 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GTP13_RS04660 ampE 951036..951890 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  GTP13_RS04665 aroP 951933..953306 (-) 1374 WP_012817732.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=359422 GTP13_RS04645 WP_000360902.1 948864..949304(-) (pilA) [Escherichia coli strain CAU16175]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=359422 GTP13_RS04645 WP_000360902.1 948864..949304(-) (pilA) [Escherichia coli strain CAU16175]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377