Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   GSY53_RS15220 Genome accession   NZ_CP047325
Coordinates   2933217..2935316 (-) Length   699 a.a.
NCBI ID   WP_159357111.1    Uniprot ID   -
Organism   Bacillus subtilis strain GOT9     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 2928217..2940316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSY53_RS15190 (GSY53_15190) spo0E 2928273..2928530 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  GSY53_RS15195 (GSY53_15195) - 2928616..2929047 (+) 432 WP_003245685.1 hypothetical protein -
  GSY53_RS15200 (GSY53_15200) kinD 2929075..2930595 (-) 1521 WP_010886497.1 sporulation kinase KinD -
  GSY53_RS15205 (GSY53_15205) mhqR 2930788..2931225 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  GSY53_RS15210 (GSY53_15210) motB 2931265..2932050 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  GSY53_RS15215 (GSY53_15215) motA 2932022..2932834 (-) 813 WP_003244739.1 flagellar motor stator protein MotA -
  GSY53_RS15220 (GSY53_15220) clpE 2933217..2935316 (-) 2100 WP_159357111.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  GSY53_RS15225 (GSY53_15225) - 2935681..2936724 (+) 1044 WP_003244712.1 hypothetical protein -
  GSY53_RS15230 (GSY53_15230) queC 2937037..2937696 (+) 660 WP_003245417.1 7-cyano-7-deazaguanine synthase QueC -
  GSY53_RS15235 (GSY53_15235) queD 2937689..2938138 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  GSY53_RS15240 (GSY53_15240) queE 2938131..2938862 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  GSY53_RS15245 (GSY53_15245) queF 2938880..2939377 (+) 498 WP_003218613.1 preQ(1) synthase -
  GSY53_RS15250 (GSY53_15250) ykvN 2939936..2940292 (-) 357 WP_003245808.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77909.95 Da        Isoelectric Point: 5.0886

>NTDB_id=359361 GSY53_RS15220 WP_159357111.1 2933217..2935316(-) (clpE) [Bacillus subtilis strain GOT9]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQTKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=359361 GSY53_RS15220 WP_159357111.1 2933217..2935316(-) (clpE) [Bacillus subtilis strain GOT9]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTTCAGACGAAAGAACAGAGCGCAGCAAAACAAAGCGAAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTAAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCTCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCATGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAGGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCAAAATAA

Domains

Predicted by InterProScan.

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.452

100

0.601
  clpC Lactococcus lactis subsp. cremoris KW2

56.557

100

0.592
  clpE Streptococcus pneumoniae TIGR4

54.785

100

0.565
  clpE Streptococcus pneumoniae Rx1

54.785

100

0.565
  clpE Streptococcus pneumoniae D39

54.785

100

0.565
  clpE Streptococcus pneumoniae R6

54.785

100

0.565
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.847

89.7

0.429
  clpC Streptococcus pneumoniae Rx1

46.25

91.559

0.423
  clpC Streptococcus pneumoniae D39

46.25

91.559

0.423
  clpC Streptococcus pneumoniae TIGR4

46.85

88.555

0.415
  clpC Streptococcus thermophilus LMD-9

46.624

88.984

0.415
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpC Streptococcus thermophilus LMG 18311

46.141

88.984

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.914

86.695

0.363

Multiple sequence alignment