Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   E8M05_RS02525 Genome accession   NZ_CP039457
Coordinates   452789..454090 (+) Length   433 a.a.
NCBI ID   WP_003063550.1    Uniprot ID   E0PC02
Organism   Streptococcus pasteurianus strain WUSP067     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447789..459090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M05_RS02500 (E8M05_02495) - 448443..449087 (+) 645 WP_013851527.1 response regulator transcription factor -
  E8M05_RS02505 (E8M05_02500) - 449205..450605 (+) 1401 WP_069789204.1 Cof-type HAD-IIB family hydrolase -
  E8M05_RS02510 (E8M05_02505) - 450605..450961 (+) 357 WP_003063541.1 S1 RNA-binding domain-containing protein -
  E8M05_RS02515 (E8M05_02510) cysK 451074..452003 (-) 930 WP_013851529.1 cysteine synthase A -
  E8M05_RS02520 (E8M05_02515) - 452107..452733 (-) 627 WP_003063547.1 YigZ family protein -
  E8M05_RS02525 (E8M05_02520) comFA/cflA 452789..454090 (+) 1302 WP_003063550.1 DEAD/DEAH box helicase Machinery gene
  E8M05_RS02530 (E8M05_02525) comFC/cflB 454087..454749 (+) 663 WP_003063553.1 ComF family protein Machinery gene
  E8M05_RS02535 (E8M05_02530) hpf 454825..455373 (+) 549 WP_003063557.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E8M05_RS11395 - 455508..456191 (+) 684 Protein_437 transposase -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49398.54 Da        Isoelectric Point: 10.0600

>NTDB_id=358781 E8M05_RS02525 WP_003063550.1 452789..454090(+) (comFA/cflA) [Streptococcus pasteurianus strain WUSP067]
MDKLNNYYGRLFTQAQLSDALKKQAKQLPAMTKLNHHYICNRCGSQVSLQNKLQTNVFYCRNCLVFGRNTSNGHLYYFPQ
RRFSKKDSLIWKGKLTPYQDDVSKGMLEGIDAKKDLLIHAVTGAGKTEMIYQSVSKIIDDGGCVCLASPRIDVCLELYKR
LSRDFSCLITLLHGDSEPYKRAPLIIATTHQLLKFYQAFDLLIIDEVDAFPFVDNAVLYHAVDRAIKPNRTKIFLTATST
DELDKKVKTGTLTKLHLARRFHANPLVVPQKIWLSGVLSNMQKKKIPQKLINLIKKQRQTHYPLLIFFPNIEQGEAFTQI
LQTYFSEEQIAFVSSKTENRLDIVEKFRRQELSILVTTTILERGVTFPCVDVFVLLSNHRLYTKSTLVQISGRVGRAAER
PTGELLFLHDGSTKEMRKAIAEIKAMNKKGGFA

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=358781 E8M05_RS02525 WP_003063550.1 452789..454090(+) (comFA/cflA) [Streptococcus pasteurianus strain WUSP067]
ATGGATAAACTGAATAATTATTATGGAAGACTATTTACACAAGCTCAACTTTCGGATGCCTTAAAAAAGCAGGCTAAACA
GTTGCCGGCAATGACAAAATTAAATCATCATTATATTTGTAATCGTTGTGGTAGTCAAGTGTCTTTACAAAATAAACTTC
AAACAAACGTTTTTTATTGTCGAAATTGCCTTGTTTTTGGAAGAAATACGAGTAATGGACATCTCTATTATTTTCCACAA
AGACGTTTTTCTAAAAAAGATTCTCTTATTTGGAAAGGAAAATTGACCCCTTACCAAGATGATGTTTCAAAAGGGATGTT
AGAAGGCATTGATGCTAAGAAAGACCTACTTATTCATGCTGTTACAGGTGCTGGAAAAACAGAAATGATTTACCAATCTG
TATCCAAAATAATTGATGACGGTGGTTGTGTTTGCCTAGCAAGTCCGCGAATTGATGTTTGTCTGGAGCTTTATAAACGC
TTGTCACGTGATTTTTCGTGTCTGATTACATTGCTTCATGGAGATTCTGAGCCCTACAAACGTGCCCCTTTAATCATTGC
CACAACACATCAACTTTTAAAATTTTACCAAGCTTTTGATTTGTTAATTATTGATGAAGTTGATGCCTTTCCATTTGTGG
ATAATGCCGTGCTTTACCATGCTGTTGATAGAGCTATCAAACCTAATAGAACCAAGATTTTTTTAACAGCGACATCAACA
GATGAATTAGATAAAAAAGTAAAAACAGGAACTCTGACTAAATTGCACCTTGCTCGACGATTTCATGCCAATCCATTAGT
TGTGCCACAAAAAATCTGGTTGTCTGGCGTTTTATCCAATATGCAAAAGAAGAAAATTCCCCAGAAATTAATAAATCTTA
TAAAAAAACAACGTCAAACCCACTATCCTTTGCTAATTTTCTTCCCAAATATCGAACAAGGAGAAGCCTTTACTCAAATT
TTACAGACCTATTTTTCAGAAGAACAAATCGCTTTTGTGTCTAGTAAAACAGAAAATCGCTTAGACATTGTAGAAAAATT
TAGGAGACAAGAACTTTCTATTTTGGTGACAACAACCATTTTAGAACGTGGTGTCACTTTCCCTTGTGTAGATGTCTTTG
TATTGCTTTCCAATCATAGATTATACACCAAAAGCACGCTAGTTCAGATTTCAGGGCGAGTTGGGAGAGCAGCAGAGCGT
CCAACAGGAGAACTGTTATTCTTGCATGACGGTAGTACCAAAGAAATGAGGAAAGCTATTGCTGAAATTAAAGCGATGAA
TAAAAAAGGAGGATTTGCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(287-397)

DEAD/DEAH box helicase (DEAD)

(96-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0PC02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

59.485

98.614

0.587
  comFA/cflA Streptococcus pneumoniae Rx1

59.485

98.614

0.587
  comFA/cflA Streptococcus pneumoniae D39

59.485

98.614

0.587
  comFA/cflA Streptococcus pneumoniae R6

59.485

98.614

0.587
  comFA/cflA Streptococcus mitis NCTC 12261

59.251

98.614

0.584
  comFA/cflA Streptococcus mitis SK321

58.548

98.614

0.577
  comFA Lactococcus lactis subsp. cremoris KW2

50

94.226

0.471
  comFA Latilactobacillus sakei subsp. sakei 23K

37.819

99.538

0.376

Multiple sequence alignment