Detailed information NTG0358355

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: SB21_RS12280
• Genome accession: NZ_CP039380
• Coordinates: 2510225..2510839 (-)
• Length: 204 a.a.
• NCBI ID: WP_020956097.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LPL-K103
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2505225..2515839

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SB21_RS12250 (SB21_12250)	rpsT	2505528..2505794 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
SB21_RS12255 (SB21_12255)	holA	2505811..2506852 (-)	1042	Protein_2348	DNA polymerase III subunit delta	-
SB21_RS12260 (SB21_12260)	-	2506910..2507043 (-)	134	Protein_2349	hypothetical protein	-
SB21_RS12265 (SB21_12265)	-	2507084..2507218 (+)	135	WP_003152870.1	YqzM family protein	-
SB21_RS12270 (SB21_12270)	comEC	2507237..2509588 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SB21_RS12275 (SB21_12275)	-	2509589..2510158 (-)	570	WP_007408264.1	ComE operon protein 2	-
SB21_RS12280 (SB21_12280)	comEA	2510225..2510839 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
SB21_RS12285 (SB21_12285)	comER	2510898..2511719 (+)	822	WP_020956098.1	late competence protein ComER	-
SB21_RS12290 (SB21_12290)	-	2511789..2512526 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
SB21_RS12295 (SB21_12295)	rsfS	2512523..2512879 (-)	357	WP_007408260.1	ribosome silencing factor	-
SB21_RS12300 (SB21_12300)	yqeK	2512897..2513457 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SB21_RS12305 (SB21_12305)	-	2513447..2514016 (-)	570	WP_039063329.1	nicotinate-nucleotide adenylyltransferase	-
SB21_RS12310 (SB21_12310)	yhbY	2514027..2514317 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
SB21_RS12315 (SB21_12315)	aroE	2514311..2515153 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22251.45 Da
• Isoelectric Point: 6.4940

>NTDB_id=358355 SB21_RS12280 WP_020956097.1 2510225..2510839(-) (comEA) [Bacillus velezensis strain LPL-K103]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=358355 SB21_RS12280 WP_020956097.1 2510225..2510839(-) (comEA) [Bacillus velezensis strain LPL-K103]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363