Detailed information NTG0358218

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E6B08_RS04685
• Genome accession: NZ_CP039371
• Coordinates: 956814..957680 (-)
• Length: 288 a.a.
• NCBI ID: WP_136912949.1
• Uniprot ID: A0A4D6X3Z0
• Organism: Pseudomonas putida strain 1290
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 951814..962680

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E6B08_RS04655 (E6B08_04655)	-	952693..953988 (-)	1296	WP_136912943.1	NAD(P)/FAD-dependent oxidoreductase	-
E6B08_RS04660 (E6B08_04660)	-	954073..954246 (-)	174	WP_136912944.1	DUF3094 family protein	-
E6B08_RS04665 (E6B08_04665)	-	954379..955008 (-)	630	WP_136912945.1	DUF1780 domain-containing protein	-
E6B08_RS04670 (E6B08_04670)	-	955113..955796 (+)	684	WP_136912946.1	energy-coupling factor ABC transporter permease	-
E6B08_RS04675 (E6B08_04675)	yacG	955994..956194 (-)	201	WP_136912947.1	DNA gyrase inhibitor YacG	-
E6B08_RS04680 (E6B08_04680)	coaE	956191..956814 (-)	624	WP_136912948.1	dephospho-CoA kinase	-
E6B08_RS04685 (E6B08_04685)	pilD	956814..957680 (-)	867	WP_136912949.1	prepilin peptidase	Machinery gene
E6B08_RS04690 (E6B08_04690)	-	957683..958891 (-)	1209	WP_136912950.1	type II secretion system F family protein	-
E6B08_RS04695 (E6B08_04695)	-	959220..959648 (+)	429	WP_136912951.1	pilin	-
E6B08_RS04705 (E6B08_04705)	-	960206..960595 (-)	390	WP_136912952.1	tautomerase family protein	-
E6B08_RS04710 (E6B08_04710)	-	960698..961540 (+)	843	WP_136912953.1	LysR family transcriptional regulator	-
E6B08_RS04715 (E6B08_04715)	-	961638..962093 (+)	456	WP_136912954.1	GFA family protein	-
E6B08_RS04720 (E6B08_04720)	-	962262..962672 (-)	411	WP_238349341.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31358.18 Da
• Isoelectric Point: 7.6213

>NTDB_id=358218 E6B08_RS04685 WP_136912949.1 956814..957680(-) (pilD) [Pseudomonas putida strain 1290]
MNAWTLLAQQPAYFITLACVLGLVVGSFLNVVVYRLPIMLQRQWQRDAQEVLGLPATEHERFDLCLPASHCPHCGQTVRA
WQNIPLLSYVALRGRCASCHTRISPRYPLVEVATALLSGVVAWHFGASAAGLLALPLTWGLLALSLIDAEHQVLPDVLVL
PMLWLGLIANAFGTYVPLADAVWGAVAGYLSLWTVYWAFKLVTGKEGMGYGDFKLLAMIGAWGGWQILPVTLVLSSLVGA
LVGLCLLRLRGRALGTAIPFGPYLAIAGWIALLWGDEMYASYLQLLGF

Nucleotide

• Length: 867 bp

>NTDB_id=358218 E6B08_RS04685 WP_136912949.1 956814..957680(-) (pilD) [Pseudomonas putida strain 1290]
ATGAATGCGTGGACCTTGCTGGCGCAACAGCCAGCCTATTTCATCACCTTGGCCTGCGTGCTCGGCCTGGTGGTGGGCAG
TTTCCTCAATGTGGTGGTGTATCGACTGCCGATCATGCTGCAGCGGCAATGGCAGCGGGACGCACAGGAGGTGCTCGGGC
TGCCGGCCACCGAGCACGAGCGCTTCGACCTCTGCTTGCCGGCTTCGCACTGCCCGCATTGTGGCCAGACGGTGCGGGCC
TGGCAGAACATCCCGCTGCTCAGCTACGTGGCCCTGCGGGGGCGGTGCGCCAGTTGCCACACGCGCATCAGCCCCCGCTA
CCCGCTGGTGGAAGTGGCCACCGCGTTGCTGTCAGGCGTGGTGGCCTGGCATTTCGGCGCCAGCGCCGCGGGGTTGCTGG
CCCTGCCATTGACCTGGGGCCTGCTGGCCTTGAGCCTGATCGACGCCGAGCACCAGGTACTGCCGGATGTGCTGGTGTTG
CCGATGCTGTGGCTGGGGCTGATCGCCAATGCCTTTGGCACCTATGTGCCGCTGGCCGATGCGGTATGGGGAGCGGTGGC
CGGGTATCTGAGCCTGTGGACGGTGTATTGGGCGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGATATGGCGACTTCA
AGCTGCTGGCGATGATCGGCGCCTGGGGTGGCTGGCAGATACTGCCGGTCACCTTGGTGCTGTCGTCGCTGGTCGGGGCG
CTGGTTGGCCTGTGCCTGTTGCGTTTGCGTGGCCGGGCACTGGGCACGGCGATACCGTTTGGGCCCTATTTGGCCATCGC
GGGGTGGATTGCGCTGCTCTGGGGTGATGAAATGTACGCCTCTTACCTGCAACTGCTTGGATTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: A0A4D6X3Z0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.986	96.181	0.5
pilD	Acinetobacter baumannii D1279779	50	95.139	0.476
pilD	Acinetobacter nosocomialis M2	50	95.139	0.476
pilD	Neisseria gonorrhoeae MS11	50.929	93.403	0.476
pilD	Vibrio campbellii strain DS40M4	48.551	95.833	0.465