Detailed information NTG0358027

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: E4T61_RS12355
• Genome accession: NZ_CP039297
• Coordinates: 2547321..2547935 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis isolate UFLA258
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2542321..2552935

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4T61_RS12325 (E4T61_12325)	rpsT	2542624..2542890 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
E4T61_RS12330 (E4T61_12330)	holA	2542907..2543948 (-)	1042	Protein_2370	DNA polymerase III subunit delta	-
E4T61_RS12335 (E4T61_12335)	-	2543988..2544139 (-)	152	Protein_2371	hypothetical protein	-
E4T61_RS12340 (E4T61_12340)	-	2544180..2544314 (+)	135	WP_003152870.1	YqzM family protein	-
E4T61_RS12345 (E4T61_12345)	comEC	2544333..2546684 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E4T61_RS12350 (E4T61_12350)	-	2546685..2547254 (-)	570	WP_094031848.1	ComE operon protein 2	-
E4T61_RS12355 (E4T61_12355)	comEA	2547321..2547935 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
E4T61_RS12360 (E4T61_12360)	comER	2547994..2548815 (+)	822	WP_012118027.1	late competence protein ComER	-
E4T61_RS12365 (E4T61_12365)	-	2548884..2549621 (-)	738	WP_136396499.1	class I SAM-dependent methyltransferase	-
E4T61_RS12370 (E4T61_12370)	rsfS	2549618..2549974 (-)	357	WP_007408260.1	ribosome silencing factor	-
E4T61_RS12375 (E4T61_12375)	yqeK	2549992..2550552 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
E4T61_RS12380 (E4T61_12380)	-	2550542..2551111 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
E4T61_RS12385 (E4T61_12385)	yhbY	2551122..2551412 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
E4T61_RS12390 (E4T61_12390)	aroE	2551406..2552248 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=358027 E4T61_RS12355 WP_043021637.1 2547321..2547935(-) (comEA) [Bacillus velezensis isolate UFLA258]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=358027 E4T61_RS12355 WP_043021637.1 2547321..2547935(-) (comEA) [Bacillus velezensis isolate UFLA258]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363