Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   E4T61_RS06005 Genome accession   NZ_CP039297
Coordinates   1159640..1161469 (+) Length   609 a.a.
NCBI ID   WP_094031915.1    Uniprot ID   -
Organism   Bacillus velezensis isolate UFLA258     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1154640..1166469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T61_RS05980 (E4T61_05980) - 1155303..1155881 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  E4T61_RS05985 (E4T61_05985) spx 1156060..1156455 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  E4T61_RS05990 (E4T61_05990) - 1156513..1157169 (-) 657 WP_007409109.1 TerC family protein -
  E4T61_RS19790 - 1157352..1157462 (+) 111 Protein_1114 adaptor protein MecA -
  E4T61_RS05995 (E4T61_05995) mecA 1157445..1158101 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  E4T61_RS06000 (E4T61_06000) - 1158252..1159412 (+) 1161 WP_059367231.1 competence protein CoiA family protein -
  E4T61_RS06005 (E4T61_06005) pepF 1159640..1161469 (+) 1830 WP_094031915.1 oligoendopeptidase F Regulator
  E4T61_RS19685 - 1161507..1161674 (-) 168 WP_003155026.1 hypothetical protein -
  E4T61_RS06010 (E4T61_06010) spxH 1161960..1162862 (-) 903 WP_136396266.1 protease adaptor protein SpxH -
  E4T61_RS06015 (E4T61_06015) - 1162859..1163257 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  E4T61_RS06020 (E4T61_06020) - 1163486..1164172 (-) 687 WP_052827260.1 lytic transglycosylase domain-containing protein -
  E4T61_RS06025 (E4T61_06025) - 1164177..1164749 (-) 573 WP_136396267.1 CYTH domain-containing protein -
  E4T61_RS06030 (E4T61_06030) - 1164874..1165239 (+) 366 WP_012117290.1 hypothetical protein -
  E4T61_RS06035 (E4T61_06035) yjbM 1165267..1165902 (+) 636 WP_007610625.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69827.48 Da        Isoelectric Point: 5.2042

>NTDB_id=358003 E4T61_RS06005 WP_094031915.1 1159640..1161469(+) (pepF) [Bacillus velezensis isolate UFLA258]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKEYMLKGLAPLGEEYASILKEGLENRWVDIYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=358003 E4T61_RS06005 WP_094031915.1 1159640..1161469(+) (pepF) [Bacillus velezensis isolate UFLA258]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAACTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAGTATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTTATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTTCTCGGCCTTGATGAAGTCCATATTTATGACCTTTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGAGTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATATATACGAAAATAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGACAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAG

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.832

97.537

0.486

Multiple sequence alignment