Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   GR131_RS12745 Genome accession   NZ_CP047147
Coordinates   2992133..2993551 (+) Length   472 a.a.
NCBI ID   WP_003948876.1    Uniprot ID   A0ABR4S2M5
Organism   Streptomyces sp. GF20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2987133..2998551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GR131_RS12720 (GR131_12720) - 2987536..2988336 (+) 801 WP_010645248.1 hypothetical protein -
  GR131_RS12725 (GR131_12725) - 2988434..2988988 (-) 555 WP_159312447.1 hypothetical protein -
  GR131_RS12730 (GR131_12730) - 2989148..2989369 (-) 222 WP_003948872.1 hypothetical protein -
  GR131_RS12735 (GR131_12735) - 2989483..2991225 (-) 1743 WP_010645242.1 sigma factor-like helix-turn-helix DNA-binding protein -
  GR131_RS12740 (GR131_12740) - 2991504..2992079 (+) 576 WP_003948874.1 dihydrofolate reductase family protein -
  GR131_RS12745 (GR131_12745) radA/sms 2992133..2993551 (+) 1419 WP_003948876.1 DNA repair protein RadA Machinery gene
  GR131_RS12750 (GR131_12750) disA 2993722..2994843 (+) 1122 WP_030770839.1 DNA integrity scanning diadenylate cyclase DisA Machinery gene
  GR131_RS12755 (GR131_12755) - 2994927..2995745 (-) 819 WP_236570758.1 hypothetical protein -
  GR131_RS12760 (GR131_12760) - 2996101..2996850 (+) 750 WP_008404973.1 phosphatase PAP2 family protein -
  GR131_RS12765 (GR131_12765) - 2996963..2998015 (+) 1053 WP_008404974.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 49821.02 Da        Isoelectric Point: 8.0022

>NTDB_id=357745 GR131_RS12745 WP_003948876.1 2992133..2993551(+) (radA/sms) [Streptomyces sp. GF20]
MAARTKSAKERPSYRCTECGWQTAKWLGRCPECQAWGTVEEYGAPAVRTTAPGRVTSSALPIAQVDGKQATARPTGVPEL
DRVLGGGLVPGAVVLVAGEPGVGKSTLLLDVAAKAASDEHRTLYVTGEESASQVRLRADRIDALSDHLYLTAETDLSAVL
GHLDAVKPSLLVLDSVQTVASPEIDGAPGGMAQVREVAGALIRASKERGMSTLLVGHVTKDGAIAGPRLLEHLVDVVLHF
EGDRHARLRLVRGVKNRYGTTDEVGCFELHDEGITGLADPSGLFLTRRAEPVPGTCLTVTLEGRRPLVAEVQALTVDSQI
PSPRRTTSGLETSRVSMMLAVLEQRGRITALGKRDIYSATVGGVKLSEPAADLAVALALASAASDTPLPKNLVAIGEVGL
AGEVRRVTGVQRRLAEAARLGFTHALVPADPGKVPPGMKVVEVADMGDALRVLPRRRRAQSPASPQEAEERR

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=357745 GR131_RS12745 WP_003948876.1 2992133..2993551(+) (radA/sms) [Streptomyces sp. GF20]
ATGGCTGCCCGTACGAAATCCGCCAAGGAACGGCCGTCCTACCGGTGCACCGAGTGCGGCTGGCAGACGGCGAAGTGGCT
CGGCCGCTGCCCCGAGTGCCAGGCCTGGGGCACGGTCGAGGAGTACGGCGCGCCCGCCGTCCGCACCACCGCCCCGGGGC
GGGTGACCAGCTCCGCCCTGCCCATCGCGCAGGTCGACGGCAAGCAGGCGACGGCCCGCCCGACCGGCGTGCCCGAGCTG
GACCGGGTGCTCGGCGGCGGTCTGGTGCCCGGCGCCGTGGTGCTGGTCGCGGGCGAGCCGGGCGTCGGCAAGTCCACGCT
GCTGCTGGACGTCGCGGCGAAGGCCGCCTCCGACGAGCACCGCACGCTGTACGTCACCGGCGAGGAGTCGGCCAGCCAGG
TCCGGCTGCGCGCCGACCGGATCGACGCGCTCAGCGACCACCTGTACCTCACCGCCGAGACCGACCTCTCCGCCGTGCTC
GGCCATCTCGACGCGGTCAAGCCCTCGTTGCTGGTCCTGGACTCCGTGCAGACCGTCGCCTCCCCCGAGATCGACGGCGC
GCCCGGCGGCATGGCGCAGGTCAGGGAGGTGGCCGGGGCGCTGATCCGGGCCTCCAAGGAGCGCGGCATGTCCACGCTCC
TGGTCGGCCACGTCACCAAGGACGGCGCCATCGCCGGCCCCCGCCTGCTGGAGCACCTGGTCGACGTGGTGCTGCACTTC
GAGGGCGACCGGCACGCCCGGCTCCGCCTGGTCCGCGGCGTCAAGAACCGCTACGGCACCACCGACGAGGTCGGCTGCTT
CGAGCTGCACGACGAGGGCATCACCGGCCTCGCCGACCCCAGCGGCCTCTTCCTGACCCGCCGCGCCGAACCCGTCCCCG
GCACCTGCCTGACGGTGACGCTGGAGGGCCGGCGCCCGCTGGTCGCCGAGGTCCAGGCGCTCACCGTCGACTCGCAGATC
CCCTCCCCTCGCCGGACCACCTCGGGGCTGGAGACCTCCCGGGTCTCGATGATGCTGGCGGTGCTGGAGCAGCGCGGCCG
CATCACCGCGCTCGGCAAGCGGGACATCTACTCGGCGACGGTCGGCGGCGTGAAGCTCTCCGAGCCCGCCGCCGACCTCG
CGGTGGCCCTCGCCCTGGCCAGCGCGGCGAGCGACACCCCGCTCCCGAAGAACCTGGTGGCGATCGGCGAGGTCGGTCTC
GCGGGCGAGGTCCGGCGGGTGACGGGCGTCCAGCGGCGGCTGGCGGAGGCGGCCCGGCTGGGCTTCACCCACGCCCTCGT
ACCGGCCGACCCGGGGAAGGTGCCGCCGGGGATGAAGGTGGTCGAAGTGGCCGACATGGGGGACGCGTTGCGGGTTCTGC
CGCGTCGGCGCCGGGCCCAGAGCCCGGCAAGCCCCCAGGAGGCGGAGGAGCGCCGGTAG

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(78-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(14-41)

Subunit ChlI of Mg-chelatase (ChlI)

(369-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

43.929

95.975

0.422
  radA Streptococcus mitis SK321

43.111

95.339

0.411
  radA Streptococcus mitis NCTC 12261

43.111

95.339

0.411
  radA Streptococcus pneumoniae Rx1

42.667

95.339

0.407
  radA Streptococcus pneumoniae D39

42.667

95.339

0.407
  radA Streptococcus pneumoniae R6

42.667

95.339

0.407
  radA Streptococcus pneumoniae TIGR4

42.667

95.339

0.407