Detailed information NTG0357178

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E5221_RS03715
• Genome accession: NZ_CP039127
• Coordinates: 785205..786071 (-)
• Length: 288 a.a.
• NCBI ID: WP_232880148.1
• Uniprot ID: -
• Organism: Pseudomonas sp. A2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 780205..791071

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5221_RS03680 (E5221_03755)	-	780550..781845 (-)	1296	WP_003260618.1	NAD(P)/FAD-dependent oxidoreductase	-
E5221_RS03685 (E5221_03760)	-	781930..782103 (-)	174	WP_013970765.1	DUF3094 family protein	-
E5221_RS03690 (E5221_03765)	-	782237..782866 (-)	630	WP_013970766.1	DUF1780 domain-containing protein	-
E5221_RS03695 (E5221_03770)	-	782922..783353 (-)	432	WP_015268860.1	hypothetical protein	-
E5221_RS03700 (E5221_03775)	-	783440..784147 (+)	708	WP_043935298.1	energy-coupling factor ABC transporter permease	-
E5221_RS03705 (E5221_03780)	yacG	784385..784585 (-)	201	WP_003260624.1	DNA gyrase inhibitor YacG	-
E5221_RS03710 (E5221_03785)	coaE	784582..785205 (-)	624	WP_013970769.1	dephospho-CoA kinase	-
E5221_RS03715 (E5221_03790)	pilD	785205..786071 (-)	867	WP_232880148.1	A24 family peptidase	Machinery gene
E5221_RS03720 (E5221_03795)	-	786074..787282 (-)	1209	WP_247846870.1	type II secretion system F family protein	-
E5221_RS03725 (E5221_03800)	-	787498..787908 (+)	411	WP_123085805.1	pilin	-
E5221_RS03735 (E5221_03810)	-	788221..789405 (+)	1185	WP_247846871.1	tyrosine-type recombinase/integrase	-
E5221_RS03740 (E5221_03815)	-	789435..790184 (+)	750	WP_157765891.1	hypothetical protein	-
E5221_RS03745 (E5221_03820)	-	790300..790503 (+)	204	WP_099593719.1	AlpA family transcriptional regulator	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31989.13 Da
• Isoelectric Point: 7.9596

>NTDB_id=357178 E5221_RS03715 WP_232880148.1 785205..786071(-) (pilD) [Pseudomonas sp. A2]
MTLWTLLAEQPAYFLTLATVLGLLVGSFLNVLVYRLPIMLERQWQREAQEMLELPITPHERFDLWLPASRCPHCAHRIRA
WENIPVISYLALRGRCSACKTRISPRYPLVEVASALLSLVVAWRFGASPEALLALPLTWCLLALSLIDAEHQLLPDALVL
PMLWLGLIVNAFGLYVPLTSALWGAVAGYLSLWTVYWVFKLATGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LAGLCLLRLRRHAMGTAIPFGPYLAIAGWIAVLWGDEIYASYTNLLGF

Nucleotide

• Length: 867 bp

>NTDB_id=357178 E5221_RS03715 WP_232880148.1 785205..786071(-) (pilD) [Pseudomonas sp. A2]
ATGACTTTATGGACTTTGCTGGCTGAGCAGCCGGCGTACTTTCTCACCCTGGCCACGGTGCTCGGCCTGCTGGTGGGCAG
TTTTCTCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAGGAAATGCTGGAAT
TGCCGATCACGCCGCACGAGCGTTTCGACCTGTGGCTACCCGCCTCGCGCTGCCCGCATTGTGCGCATCGGATTCGCGCA
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGCGCGGGCGTTGTTCAGCCTGCAAAACCCGCATCAGCCCGCGCTA
CCCGTTGGTGGAGGTGGCCAGCGCGCTGCTGTCGCTGGTGGTGGCCTGGCGCTTTGGTGCTTCGCCCGAGGCCTTGCTGG
CGCTGCCGCTGACCTGGTGCCTGCTGGCCCTGAGCCTGATCGATGCCGAGCACCAGTTGCTGCCGGATGCGCTGGTGCTG
CCGATGCTGTGGCTGGGGCTGATCGTCAACGCCTTTGGCCTCTACGTCCCGCTGACCAGTGCGTTGTGGGGCGCGGTGGC
CGGATACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAAGCTGGCCACTGGCAAGGAAGGCATGGGCTATGGCGACTTCA
AGCTGATGGCGCTGATCGGGGCCTGGGGTGGCTGGCAGGTGTTGCCATTGACCTTGCTGTTGTCGTCAGTGGTCGGGGCG
CTGGCTGGGCTGTGCCTGTTGCGCTTGCGCCGCCATGCGATGGGCACGGCGATCCCGTTTGGCCCCTATCTGGCGATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCTTCATACACAAACCTGCTTGGATTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.545	95.486	0.521
pilD	Acinetobacter baumannii D1279779	52.143	97.222	0.507
pilD	Acinetobacter nosocomialis M2	51.429	97.222	0.5
pilD	Vibrio campbellii strain DS40M4	50.178	97.569	0.49
pilD	Neisseria gonorrhoeae MS11	51.321	92.014	0.472