Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RI02_RS11140 Genome accession   NZ_CP039020
Coordinates   2189963..2191033 (-) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain BA06     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2184963..2196033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI02_RS11090 (RI02_11185) sipW 2185062..2185634 (-) 573 WP_017357979.1 signal peptidase I SipW -
  RI02_RS11095 (RI02_11190) tapA 2185685..2186212 (-) 528 WP_017367589.1 amyloid fiber anchoring/assembly protein TapA -
  RI02_RS11100 (RI02_11195) - 2186465..2186791 (+) 327 WP_017367590.1 DUF3889 domain-containing protein -
  RI02_RS11105 (RI02_11200) - 2186827..2187015 (-) 189 WP_017367591.1 YqzE family protein -
  RI02_RS11110 (RI02_11205) - 2187076..2187459 (-) 384 WP_017357982.1 competence type IV pilus minor pilin ComGG -
  RI02_RS11115 (RI02_11210) comGF 2187456..2187953 (-) 498 WP_017367592.1 competence type IV pilus minor pilin ComGF -
  RI02_RS11120 (RI02_11215) comGE 2187895..2188209 (-) 315 WP_017367593.1 competence type IV pilus minor pilin ComGE -
  RI02_RS11125 (RI02_11220) comGD 2188193..2188639 (-) 447 WP_017367594.1 competence type IV pilus minor pilin ComGD -
  RI02_RS11130 (RI02_11225) comGC 2188632..2188925 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  RI02_RS11135 (RI02_11230) comGB 2188942..2189982 (-) 1041 WP_017367595.1 competence type IV pilus assembly protein ComGB -
  RI02_RS11140 (RI02_11235) comGA 2189963..2191033 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  RI02_RS11145 (RI02_11240) - 2191491..2192360 (-) 870 WP_017367596.1 STAS domain-containing protein -
  RI02_RS11155 (RI02_11250) - 2192586..2192966 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RI02_RS11160 (RI02_11255) - 2193179..2193421 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  RI02_RS11165 (RI02_11260) - 2193470..2194108 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  RI02_RS11170 (RI02_11265) - 2194269..2194442 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  RI02_RS11175 (RI02_11270) - 2194467..2194781 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=356728 RI02_RS11140 WP_017357987.1 2189963..2191033(-) (comGA) [Bacillus altitudinis strain BA06]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=356728 RI02_RS11140 WP_017357987.1 2189963..2191033(-) (comGA) [Bacillus altitudinis strain BA06]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAGGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTACAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGAGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649

Multiple sequence alignment