Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GPY01_RS11535 Genome accession   NZ_CP046839
Coordinates   1549532..1551259 (-) Length   575 a.a.
NCBI ID   WP_123161483.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2011EL-1271     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1544532..1556259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY01_RS11500 - 1544800..1545579 (-) 780 WP_001963697.1 type II secretion system protein -
  GPY01_RS11505 - 1545579..1546145 (-) 567 WP_184480124.1 type II secretion system protein -
  GPY01_RS11510 - 1546135..1546647 (-) 513 WP_001249940.1 type II secretion system protein -
  GPY01_RS11515 - 1546710..1547174 (-) 465 WP_000828590.1 type II secretion system protein -
  GPY01_RS11520 - 1547209..1547763 (-) 555 WP_000738707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY01_RS11525 - 1547845..1548297 (-) 453 WP_000689652.1 hypothetical protein -
  GPY01_RS11530 - 1548300..1549523 (-) 1224 WP_123161482.1 type II secretion system F family protein -
  GPY01_RS11535 pilB/pilB1 1549532..1551259 (-) 1728 WP_123161483.1 GspE/PulE family protein Machinery gene
  GPY01_RS11540 - 1551249..1552400 (-) 1152 WP_033930070.1 tetratricopeptide repeat protein -
  GPY01_RS11545 - 1552397..1553242 (-) 846 WP_000281359.1 ExeA family protein -
  GPY01_RS11550 mshL 1553247..1554926 (-) 1680 WP_032471340.1 pilus (MSHA type) biogenesis protein MshL -
  GPY01_RS11555 - 1554941..1555258 (-) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  GPY01_RS11560 gspM 1555251..1555901 (-) 651 WP_000835150.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63829.27 Da        Isoelectric Point: 5.8021

>NTDB_id=356332 GPY01_RS11535 WP_123161483.1 1549532..1551259(-) (pilB/pilB1) [Vibrio cholerae strain 2011EL-1271]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQYGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYSGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=356332 GPY01_RS11535 WP_123161483.1 1549532..1551259(-) (pilB/pilB1) [Vibrio cholerae strain 2011EL-1271]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTCAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCGGTGATCGATTTGAGCCGCGCTCATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTTTCGTTCGCCGAACAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACTGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTATTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAGTTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGTACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCGGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCCGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTCGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTTCGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGTCAAAACTGTAACTTAACTGGTTATAGCGGCCGGATCGGTGTATTTGA
AATGCTTGAACTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTGCAAGGCATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.762

100

0.376
  pilB Vibrio campbellii strain DS40M4

36.972

98.783

0.365
  pilB Vibrio cholerae strain A1552

37.123

97.913

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.658

96.522

0.363
  pilF Thermus thermophilus HB27

37.388

97.217

0.363
  pilB Vibrio parahaemolyticus RIMD 2210633

36.189

99.478

0.36