Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VEA_RS03725 Genome accession   NC_013456
Coordinates   778947..779963 (+) Length   338 a.a.
NCBI ID   WP_005395718.1    Uniprot ID   A0AAE4S9C7
Organism   Vibrio antiquarius     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 773947..784963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEA_RS03710 (VEA_002319) - 774380..775108 (-) 729 WP_005395721.1 redoxin family protein -
  VEA_RS03715 (VEA_002320) oxyR 775279..776190 (+) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  VEA_RS03720 (VEA_002321) - 776242..778803 (-) 2562 WP_006742776.1 PBP1A family penicillin-binding protein -
  VEA_RS03725 (VEA_002322) pilM 778947..779963 (+) 1017 WP_005395718.1 type IV pilus assembly protein PilM Machinery gene
  VEA_RS03730 (VEA_002323) pilN 779947..780522 (+) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  VEA_RS03735 (VEA_002324) pilO 780515..781108 (+) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  VEA_RS03740 (VEA_002325) pilP 781098..781613 (+) 516 WP_012841352.1 pilus assembly protein PilP Machinery gene
  VEA_RS03745 (VEA_002326) pilQ 781639..783387 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ family protein Machinery gene
  VEA_RS03750 (VEA_002327) aroK 783575..784093 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.08 Da        Isoelectric Point: 6.9711

>NTDB_id=35626 VEA_RS03725 WP_005395718.1 778947..779963(+) (pilM) [Vibrio antiquarius]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=35626 VEA_RS03725 WP_005395718.1 778947..779963(+) (pilM) [Vibrio antiquarius]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAATTGCAAATAGAACAAAACCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTTACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTGTTTCACAAGGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTGCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATTGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACTTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment