Detailed information NTG0356097

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: GPY55_RS23845
• Genome accession: NZ_CP046831
• Coordinates: 2888609..2890813 (+)
• Length: 734 a.a.
• NCBI ID: WP_029805229.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain 2012AW-0224
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2883609..2895813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPY55_RS23800	rplV	2884278..2884610 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
GPY55_RS23805	rpsS	2884621..2884899 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
GPY55_RS23810	rplB	2884921..2885745 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
GPY55_RS23815	rplW	2885761..2886063 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
GPY55_RS23820	rplD	2886060..2886662 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
GPY55_RS23825	rplC	2886680..2887309 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
GPY55_RS23830	rpsJ	2887324..2887635 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
GPY55_RS23835	rpmE	2888093..2888314 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
GPY55_RS23845	priA	2888609..2890813 (+)	2205	WP_029805229.1	primosomal protein N'	Machinery gene
GPY55_RS23850	cytR	2891146..2892153 (+)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
GPY55_RS23855	ftsN	2892330..2892875 (+)	546	WP_005481405.1	cell division protein FtsN	-
GPY55_RS23860	hslV	2893040..2893591 (+)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
GPY55_RS23865	hslU	2893615..2894946 (+)	1332	WP_005489452.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82299.93 Da
• Isoelectric Point: 8.7811

>NTDB_id=356097 GPY55_RS23845 WP_029805229.1 2888609..2890813(+) (priA) [Vibrio parahaemolyticus strain 2012AW-0224]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWRITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITKWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=356097 GPY55_RS23845 WP_029805229.1 2888609..2890813(+) (priA) [Vibrio parahaemolyticus strain 2012AW-0224]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGTGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCGAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCAA
GTGGGGGCAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTTAACCTGATCAAA
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAACGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGATCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGTTCACAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAATTATTTCCCGAATACAA
AGCCATCCGTATCGACAGGGATAGTACGCGCCGCAAAGGTAGCTTAGAAGACGCGTTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCCCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTAGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTACAGACTCACCATCCTGAGCACAGTTTATTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCTCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.419	100	0.436
priA	Bacillus subtilis subsp. subtilis str. 168	34.131	100	0.369