Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY32_RS12920 Genome accession   NZ_CP046828
Coordinates   828158..828610 (-) Length   150 a.a.
NCBI ID   WP_005493986.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2010V-1106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823158..833610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY32_RS12900 coaE 823675..824289 (-) 615 WP_005493969.1 dephospho-CoA kinase -
  GPY32_RS12905 pilD 824290..825159 (-) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GPY32_RS12910 pilC 825224..826447 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GPY32_RS12915 pilB 826471..828156 (-) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY32_RS12920 pilA 828158..828610 (-) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY32_RS12925 nadC 828874..829761 (-) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY32_RS12930 ampD 829854..830405 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY32_RS12935 pdhR 830811..831578 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15708.99 Da        Isoelectric Point: 7.1316

>NTDB_id=355980 GPY32_RS12920 WP_005493986.1 828158..828610(-) (pilA) [Vibrio parahaemolyticus strain 2010V-1106]
MKHSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPDSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=355980 GPY32_RS12920 WP_005493986.1 828158..828610(-) (pilA) [Vibrio parahaemolyticus strain 2010V-1106]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTGATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

42.105

88.667

0.373