Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GP469_RS10575 Genome accession   NZ_CP046808
Coordinates   2099879..2100331 (-) Length   150 a.a.
NCBI ID   WP_005493986.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2013V-1146     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2094879..2105331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP469_RS10555 coaE 2095396..2096010 (-) 615 WP_005493969.1 dephospho-CoA kinase -
  GP469_RS10560 pilD 2096011..2096880 (-) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GP469_RS10565 pilC 2096945..2098168 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GP469_RS10570 pilB 2098192..2099877 (-) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GP469_RS10575 pilA 2099879..2100331 (-) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GP469_RS10580 nadC 2100595..2101482 (-) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GP469_RS10585 ampD 2101575..2102126 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GP469_RS10590 pdhR 2102532..2103299 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15708.99 Da        Isoelectric Point: 7.1316

>NTDB_id=355923 GP469_RS10575 WP_005493986.1 2099879..2100331(-) (pilA) [Vibrio parahaemolyticus strain 2013V-1146]
MKHSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPDSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=355923 GP469_RS10575 WP_005493986.1 2099879..2100331(-) (pilA) [Vibrio parahaemolyticus strain 2013V-1146]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTGATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

42.105

88.667

0.373