Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY34_RS20180 Genome accession   NZ_CP046778
Coordinates   2312724..2313176 (-) Length   150 a.a.
NCBI ID   WP_005493986.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2014V-1125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2307724..2318176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY34_RS20160 coaE 2308241..2308855 (-) 615 WP_005493969.1 dephospho-CoA kinase -
  GPY34_RS20165 pilD 2308856..2309725 (-) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GPY34_RS20170 pilC 2309790..2311013 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GPY34_RS20175 pilB 2311037..2312722 (-) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY34_RS20180 pilA 2312724..2313176 (-) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY34_RS20185 nadC 2313440..2314327 (-) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY34_RS20190 ampD 2314420..2314971 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY34_RS20195 pdhR 2315377..2316144 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15708.99 Da        Isoelectric Point: 7.1316

>NTDB_id=355403 GPY34_RS20180 WP_005493986.1 2312724..2313176(-) (pilA) [Vibrio parahaemolyticus strain 2014V-1125]
MKHSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPDSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=355403 GPY34_RS20180 WP_005493986.1 2312724..2313176(-) (pilA) [Vibrio parahaemolyticus strain 2014V-1125]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTGATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

42.105

88.667

0.373