Detailed information NTG0354434

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E4188_RS05225
• Genome accession: NZ_CP038448
• Coordinates: 1114529..1115401 (+)
• Length: 290 a.a.
• NCBI ID: WP_042649027.1
• Uniprot ID: -
• Organism: Aeromonas media strain R50-22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1109529..1120401

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4188_RS05205 (E4188_05275)	tnpA	1110308..1110733 (+)	426	WP_041205574.1	IS200/IS605-like element ISAeme8 family transposase	-
E4188_RS23970	-	1110906..1111073 (+)	168	Protein_1009	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E4188_RS05215 (E4188_05290)	pilB	1111371..1113077 (+)	1707	WP_042649025.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
E4188_RS05220 (E4188_05295)	pilC	1113207..1114448 (+)	1242	WP_042649026.1	type II secretion system F family protein	Machinery gene
E4188_RS05225 (E4188_05300)	pilD	1114529..1115401 (+)	873	WP_042649027.1	A24 family peptidase	Machinery gene
E4188_RS05230 (E4188_05305)	coaE	1115420..1116034 (+)	615	WP_042649028.1	dephospho-CoA kinase	-
E4188_RS05235 (E4188_05310)	zapD	1116072..1116794 (+)	723	WP_042649029.1	cell division protein ZapD	-
E4188_RS05240 (E4188_05315)	yacG	1116804..1116998 (+)	195	WP_042649030.1	DNA gyrase inhibitor YacG	-
E4188_RS05245 (E4188_05320)	mutT	1117074..1117478 (-)	405	WP_005332118.1	8-oxo-dGTP diphosphatase MutT	-
E4188_RS05250 (E4188_05325)	secA	1117634..1120354 (-)	2721	WP_042649031.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32052.32 Da
• Isoelectric Point: 7.0918

>NTDB_id=354434 E4188_RS05225 WP_042649027.1 1114529..1115401(+) (pilD) [Aeromonas media strain R50-22]
MVPLIELAQGLPWLYLSLIFLFSLMIGSFLNVVIHRLPIMLEREWHADYRDYFGSEVATSPAEPYNLMVPRSRCPHCDHP
ISALENIPLLSWLWLKGRCRQCKAPISARYPLVELLTALLSVTVAATLTPGWGALAALALTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLAGGFVPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGHGDFKLLAALGAWLGWQALPIVLLLSSL
VGAIIGIGLILLRNHHQGKPIPFGPYLAIAGWIALLWGDNITRWYLDIVL

Nucleotide

• Length: 873 bp

>NTDB_id=354434 E4188_RS05225 WP_042649027.1 1114529..1115401(+) (pilD) [Aeromonas media strain R50-22]
ATGGTGCCTCTGATCGAACTGGCCCAGGGCCTGCCCTGGCTCTATCTCTCCCTGATTTTTCTCTTCTCCCTGATGATTGG
CAGCTTCCTTAACGTGGTGATCCATCGCCTGCCCATCATGCTGGAGCGGGAGTGGCATGCAGACTACCGTGATTACTTCG
GCAGCGAGGTTGCCACTTCCCCCGCCGAACCTTACAACCTGATGGTACCGCGCTCTCGCTGCCCCCACTGCGATCACCCC
ATAAGCGCGCTGGAGAACATCCCCCTGCTGAGCTGGCTCTGGCTCAAGGGACGTTGCCGACAATGCAAGGCGCCAATTTC
GGCCCGCTATCCCCTGGTCGAGCTGCTGACCGCCCTGTTGTCGGTCACCGTTGCGGCGACCCTCACTCCCGGCTGGGGAG
CCCTGGCAGCCCTCGCACTGACCTGGGTACTGGTGGCCCTCACCTTCATCGATCTGGACAAGATGCTGCTGCCGGACCAG
CTCACACTGCCGCTGCTGTGGGGCGGTCTGTTGTTCAATCTGGCAGGCGGCTTTGTGCCGCTGGCCGATGCGGTGATCGG
CGCCATGGCTGGCTATCTGGTGCTCTGGAGTCTCTACTGGGCGTTCAAGCTGCTGACCGGCAAGGAGGGGATGGGTCATG
GCGACTTCAAGCTGCTGGCGGCACTCGGTGCCTGGCTGGGCTGGCAGGCGCTGCCCATAGTGCTGCTGCTCTCTTCACTG
GTCGGCGCCATCATCGGGATCGGACTTATCCTGCTGCGCAACCATCATCAGGGCAAACCCATCCCCTTTGGCCCCTATCT
CGCCATCGCGGGCTGGATAGCACTGCTGTGGGGGGATAACATCACCCGCTGGTATCTGGACATCGTTCTCTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

• Type IV leader peptidase family (Peptidase_A24) (139-247)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	61.404	98.276	0.603
pilD	Vibrio campbellii strain DS40M4	56.055	99.655	0.559
pilD	Acinetobacter nosocomialis M2	52.518	95.862	0.503
pilD	Acinetobacter baumannii D1279779	52.158	95.862	0.5
pilD	Neisseria gonorrhoeae MS11	49.049	90.69	0.445