Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   E4T63_RS23880 Genome accession   NZ_CP038438
Coordinates   5176451..5178040 (+) Length   529 a.a.
NCBI ID   WP_135296951.1    Uniprot ID   A0AAP8Z6X6
Organism   Pseudomonas fluorescens strain LBUM677     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5171451..5183040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS23860 (E4T63_23860) pgeF 5173246..5173968 (-) 723 WP_134787357.1 peptidoglycan editing factor PgeF -
  E4T63_RS23865 (E4T63_23865) rluD 5173965..5174927 (-) 963 WP_007963852.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  E4T63_RS23870 (E4T63_23870) - 5175079..5176101 (+) 1023 WP_027612809.1 outer membrane protein assembly factor BamD -
  E4T63_RS23875 (E4T63_23875) - 5176228..5176461 (+) 234 WP_045122641.1 PP0621 family protein -
  E4T63_RS23880 (E4T63_23880) pilS 5176451..5178040 (+) 1590 WP_135296951.1 ATP-binding protein Regulator
  E4T63_RS23885 (E4T63_23885) pilR 5178037..5179383 (+) 1347 WP_134787358.1 sigma-54 dependent transcriptional regulator Regulator
  E4T63_RS23890 (E4T63_23890) thiO 5179515..5180615 (-) 1101 WP_135296587.1 glycine oxidase ThiO -
  E4T63_RS23895 (E4T63_23895) - 5180675..5181076 (-) 402 WP_135296588.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58595.30 Da        Isoelectric Point: 7.0069

>NTDB_id=354292 E4T63_RS23880 WP_135296951.1 5176451..5178040(+) (pilS) [Pseudomonas fluorescens strain LBUM677]
MIAEAANADRKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSADDDLLRSGSWLYLVLNILLVVFLENTRRPAQLF
TLALVDVLLLCSLFYAAGGVASAIGNLLIVSVAIANTLLRRRIGLLIAAIGALGIVGSSFLLGFSHPLSANDYLQAGTLG
ALCFAASLLVQGLIRRLEVSENLAEQRASEVTSLEALNALILQRMRTGILVLDEQRRVQLANHSAKNLLAQSHLEGHLIS
DYSPALVERLQLWLSNPTLRPQSLKIPGNGLELQPSFIALDQSPNQQTLVFLEDLAQIAQQAQQLKLAALGRLTAGISHE
IRNPLGAISHAAQLLAESEELDSADRRLTQIIQDHSRRMNRVIENVLQLSRRQQSAPQRLDLKPWLEHFVAESREQASER
QSIHLRINSGDFITLMDPDQLTQILDNLLRNGWRHSVLLHQQAEVWLALFVDPDSQLAVLEVQDNGPGVPSDQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=354292 E4T63_RS23880 WP_135296951.1 5176451..5178040(+) (pilS) [Pseudomonas fluorescens strain LBUM677]
GTGATCGCTGAGGCCGCCAACGCCGACCGTAAACAGGCGCAGCGCCTGCTGCGCCTCTATCATCTCTACCGTTTAAGCGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACGTCAGCCGACGACGATCTGCTGCGCA
GCGGCAGTTGGTTGTATCTGGTGCTGAACATTCTGCTGGTGGTGTTCCTGGAGAACACCCGCCGCCCGGCGCAGTTGTTC
ACCCTGGCCTTGGTCGATGTGCTGTTGCTGTGCAGTCTGTTCTACGCGGCGGGCGGCGTGGCCAGCGCCATCGGCAACCT
GCTGATTGTCTCGGTGGCGATCGCCAACACCCTGCTGCGCCGGCGCATCGGCCTGCTGATTGCCGCCATCGGCGCACTCG
GCATAGTCGGCTCCAGCTTTCTGCTGGGCTTCAGTCACCCCCTGAGCGCCAATGACTATCTGCAAGCCGGGACCCTCGGC
GCTTTGTGTTTTGCCGCATCGTTGCTGGTCCAGGGCCTGATCCGGCGTCTGGAAGTCAGCGAAAATCTCGCCGAACAGCG
TGCCAGCGAAGTCACCAGTCTTGAAGCCCTCAACGCCCTGATTCTGCAACGCATGCGCACCGGCATCCTGGTACTCGACG
AACAGCGCCGGGTACAGCTGGCCAATCACAGCGCGAAAAATCTGTTGGCCCAGTCGCATCTTGAAGGGCATTTGATCAGC
GACTACTCGCCGGCATTAGTCGAGCGTCTGCAGCTGTGGCTGAGCAACCCGACCCTGCGCCCGCAGAGTCTGAAGATCCC
CGGCAACGGCCTGGAACTGCAACCCAGCTTCATCGCCCTCGACCAGAGTCCCAACCAGCAAACCCTGGTGTTCCTCGAAG
ATCTGGCGCAAATCGCTCAGCAAGCCCAGCAGCTCAAACTCGCCGCACTGGGGCGTCTGACTGCCGGCATCTCCCATGAA
ATCCGCAATCCACTGGGCGCCATCAGTCATGCCGCGCAGCTATTGGCGGAGTCCGAGGAACTCGATAGCGCCGATCGGCG
TCTGACGCAGATCATTCAAGACCACTCCCGCCGCATGAACCGAGTCATCGAAAACGTCCTGCAACTGTCGCGCCGCCAGC
AAAGCGCTCCGCAACGGCTGGATCTCAAACCGTGGCTGGAACACTTTGTTGCCGAGAGCCGCGAGCAGGCGAGCGAGCGC
CAGTCGATTCATTTGCGGATCAATTCCGGGGACTTCATCACGTTGATGGATCCCGACCAGCTCACGCAGATTCTCGACAA
TCTGTTACGCAACGGCTGGCGGCACAGCGTTCTGCTGCACCAGCAGGCCGAAGTCTGGCTGGCGCTGTTCGTCGACCCCG
ACAGCCAGCTGGCGGTGCTCGAAGTACAGGACAACGGCCCCGGTGTGCCGTCCGATCAGCAGGCGCATCTGTTCGAACCG
TTCTTCACCACCAGCAGCCAAGGCACCGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCGCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.434

100

0.595

Multiple sequence alignment