Detailed information NTG0353096

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E4K08_RS20240
• Genome accession: NZ_CP038281
• Coordinates: 3495174..3495971 (-)
• Length: 265 a.a.
• NCBI ID: WP_088911853.1
• Uniprot ID: -
• Organism: Raoultella ornithinolytica strain WLK218
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3490174..3500971

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4K08_RS20220 (E4K08_20345)	dksA	3490779..3491234 (-)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-
E4K08_RS20225 (E4K08_20350)	sfsA	3491399..3492115 (-)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
E4K08_RS20230 (E4K08_20355)	thpR	3492115..3492651 (-)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
E4K08_RS20235 (E4K08_20360)	hrpB	3492724..3495153 (+)	2430	WP_088402374.1	ATP-dependent helicase HrpB	-
E4K08_RS20240 (E4K08_20365)	pilD	3495174..3495971 (-)	798	WP_088911853.1	prepilin peptidase	Machinery gene
E4K08_RS20245 (E4K08_20370)	-	3495986..3496726 (-)	741	WP_032716580.1	type II secretion system protein N	-
E4K08_RS20250 (E4K08_20375)	gspM	3496716..3497210 (-)	495	WP_064357507.1	type II secretion system protein GspM	-
E4K08_RS20255 (E4K08_20380)	gspL	3497203..3498405 (-)	1203	WP_004858036.1	type II secretion system protein GspL	-
E4K08_RS20260 (E4K08_20385)	gspK	3498392..3499378 (-)	987	WP_088911851.1	type II secretion system minor pseudopilin GspK	-
E4K08_RS20265 (E4K08_20390)	gspJ	3499375..3499959 (-)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
E4K08_RS20270 (E4K08_20395)	gspI	3499971..3500336 (-)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
E4K08_RS20275 (E4K08_20400)	gspH	3500333..3500818 (-)	486	WP_233471560.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29348.01 Da
• Isoelectric Point: 8.4762

>NTDB_id=353096 E4K08_RS20240 WP_088911853.1 3495174..3495971(-) (pilD) [Raoultella ornithinolytica strain WLK218]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFMLAGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSSLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=353096 E4K08_RS20240 WP_088911853.1 3495174..3495971(-) (pilD) [Raoultella ornithinolytica strain WLK218]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCTTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAGCTGGCCAGCGGGCTGCTCTTTATGTT
GGCCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGCGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCAGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.275	96.226	0.445
pilD	Acinetobacter baumannii D1279779	44.574	97.358	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43