Detailed information NTG0352963

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: SaO331_RS07590
• Genome accession: NZ_CP038269
• Coordinates: 1537750..1538247 (-)
• Length: 165 a.a.
• NCBI ID: WP_001838632.1
• Uniprot ID: A0A6A9GZK7
• Organism: Staphylococcus aureus strain O331 isolate B114
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1532750..1543247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SaO331_RS07565 (SaO331_1394)	gcvPB	1532912..1534384 (-)	1473	WP_000202177.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
SaO331_RS07570 (SaO331_1395)	gcvPA	1534377..1535723 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
SaO331_RS07575 (SaO331_1396)	gcvT	1535743..1536834 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
SaO331_RS07580 (SaO331_1397)	-	1536993..1537517 (-)	525	WP_001015116.1	shikimate kinase	-
SaO331_RS07585	-	1537507..1537653 (-)	147	WP_001789879.1	hypothetical protein	-
SaO331_RS07590 (SaO331_1398)	comGF	1537750..1538247 (-)	498	WP_001838632.1	competence type IV pilus minor pilin ComGF	Machinery gene
SaO331_RS07595 (SaO331_1399)	comGE	1538165..1538464 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
SaO331_RS07600 (SaO331_1400)	comGD	1538451..1538897 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
SaO331_RS07605 (SaO331_1401)	comGC	1538875..1539186 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
SaO331_RS07610 (SaO331_1402)	comGB	1539200..1540270 (-)	1071	WP_000776407.1	competence type IV pilus assembly protein ComGB	Machinery gene
SaO331_RS07615 (SaO331_1403)	comGA	1540242..1541216 (-)	975	WP_000697218.1	competence type IV pilus ATPase ComGA	Machinery gene
SaO331_RS07620 (SaO331_1404)	-	1541268..1541891 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
SaO331_RS07625 (SaO331_1405)	-	1541888..1542217 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
SaO331_RS07630 (SaO331_1406)	-	1542217..1543203 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19184.87 Da
• Isoelectric Point: 10.2192

>NTDB_id=352963 SaO331_RS07590 WP_001838632.1 1537750..1538247(-) (comGF) [Staphylococcus aureus strain O331 isolate B114]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIENREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=352963 SaO331_RS07590 WP_001838632.1 1537750..1538247(-) (comGF) [Staphylococcus aureus strain O331 isolate B114]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCTCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAAATAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A6A9GZK7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	96.364	100	0.964
comGF	Staphylococcus aureus MW2	96.364	100	0.964