Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EXH44_RS07670 Genome accession   NZ_CP038145
Coordinates   1546359..1546934 (-) Length   191 a.a.
NCBI ID   WP_162857554.1    Uniprot ID   A0A4P7CMD1
Organism   Actinobacillus indolicus strain AIFJ1607     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1541359..1551934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXH44_RS07635 (EXH44_07635) - 1541495..1542148 (+) 654 WP_162856388.1 IS1595 family transposase -
  EXH44_RS07640 (EXH44_07640) - 1542168..1542518 (-) 351 WP_135674715.1 DsrE family protein -
  EXH44_RS07645 (EXH44_07645) - 1542844..1543008 (-) 165 WP_162856943.1 Trm112 family protein -
  EXH44_RS07650 (EXH44_07650) lpxK 1542989..1543990 (-) 1002 WP_162856944.1 tetraacyldisaccharide 4'-kinase -
  EXH44_RS07655 (EXH44_07655) clpX 1544034..1545284 (-) 1251 WP_021117767.1 ATP-dependent protease ATP-binding subunit ClpX -
  EXH44_RS07660 (EXH44_07660) clpP 1545291..1545872 (-) 582 WP_162856945.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  EXH44_RS07665 (EXH44_07665) - 1545971..1546366 (+) 396 WP_162856946.1 SufE family protein -
  EXH44_RS07670 (EXH44_07670) pilF 1546359..1546934 (-) 576 WP_162857554.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EXH44_RS07675 (EXH44_07675) - 1547065..1548225 (-) 1161 WP_162856947.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EXH44_RS07700 (EXH44_07700) gltX 1549098..1550537 (+) 1440 WP_162856948.1 glutamate--tRNA ligase -
  EXH44_RS07710 (EXH44_07710) - 1550940..1551728 (+) 789 WP_162856949.1 glycosyltransferase -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 21802.64 Da        Isoelectric Point: 8.0908

>NTDB_id=351770 EXH44_RS07670 WP_162857554.1 1546359..1546934(-) (pilF) [Actinobacillus indolicus strain AIFJ1607]
MTFAKFFRNLTACSVALWLVACTNQTAKVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDAKDYLPHSVLAYY
YQQTGDSPKAEEAYQQALKLSKTQSKSNQFRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MEKNVAKQNEALEALAKLDQTRADKLRPLFK

Nucleotide


Download         Length: 576 bp        

>NTDB_id=351770 EXH44_RS07670 WP_162857554.1 1546359..1546934(-) (pilF) [Actinobacillus indolicus strain AIFJ1607]
ATGACCTTCGCAAAATTTTTCCGTAATTTGACCGCTTGTAGTGTGGCGTTATGGCTTGTTGCTTGTACAAATCAAACCGC
AAAGGTGGATTTTAACCGTTCAGAAGCCGTCAAAGCTCGCATTAATTTAGCACTGGCTTACCTTGAGCAATCTGATTTTC
CAAAAGCGAAAGAGAACATCGACAAAGCGCTTGAACACGATGCAAAAGACTATTTACCGCACTCTGTACTTGCCTATTAT
TACCAACAAACCGGCGATAGCCCAAAAGCAGAAGAAGCCTACCAACAAGCCCTGAAGTTAAGTAAAACACAGAGCAAAAG
CAACCAATTTCGTCCCGATGTATTGAATAACTACGGTACCTTTCTCTGTAAACAAAAGCAATTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTATAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGGAAAAGAATGTGGCTAAACAAAATGAGGCTTTAGAGGCTTTGGCGAAATTAGATCAAACAAGGGCAGATAAACTTCG
CCCGCTTTTTAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(77-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7CMD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

87.634

97.382

0.853
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.055

95.288

0.429

Multiple sequence alignment