Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E2R23_RS18960 Genome accession   NZ_CP037970
Coordinates   549536..550855 (-) Length   439 a.a.
NCBI ID   WP_028358576.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain BPs123     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544536..555855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2R23_RS18940 (E2R23_18940) - 545435..545830 (-) 396 WP_004530943.1 nitrite reductase (NAD(P)H) small subunit -
  E2R23_RS18945 (E2R23_18945) nirB 545906..548350 (-) 2445 WP_004522864.1 nitrite reductase large subunit NirB -
  E2R23_RS18950 (E2R23_18950) - 548397..548738 (+) 342 WP_009918580.1 hypothetical protein -
  E2R23_RS18955 (E2R23_18955) maiA 548735..549379 (+) 645 WP_004548087.1 maleylacetoacetate isomerase -
  E2R23_RS18960 (E2R23_18960) pilA 549536..550855 (-) 1320 WP_028358576.1 signal recognition particle-docking protein FtsY Machinery gene
  E2R23_RS18965 (E2R23_18965) rsmD 551107..551733 (+) 627 WP_004522861.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  E2R23_RS18975 (E2R23_18975) coaD 551844..552344 (+) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  E2R23_RS18980 (E2R23_18980) - 552407..552673 (+) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  E2R23_RS18985 (E2R23_18985) hisC 552843..553913 (-) 1071 WP_004553797.1 histidinol-phosphate transaminase -
  E2R23_RS18990 (E2R23_18990) pth 554002..554607 (-) 606 WP_004202941.1 aminoacyl-tRNA hydrolase -
  E2R23_RS18995 (E2R23_18995) - 554721..555335 (-) 615 WP_004200150.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46384.69 Da        Isoelectric Point: 6.0733

>NTDB_id=351083 E2R23_RS18960 WP_028358576.1 549536..550855(-) (pilA) [Burkholderia pseudomallei strain BPs123]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDERSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLGALREKVRAGRLTDPQQVKDALRELLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=351083 E2R23_RS18960 WP_028358576.1 549536..550855(-) (pilA) [Burkholderia pseudomallei strain BPs123]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCACCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCGCA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAGCCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCGTCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGATGCGCTGCGCGAGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCCGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTTCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(158-219)

SRP54-type protein, GTPase domain (SRP54)

(240-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.339

100

0.508

Multiple sequence alignment