Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HPF_RS21815 Genome accession   NZ_CP037867
Coordinates   4532210..4533829 (-) Length   539 a.a.
NCBI ID   WP_133157812.1    Uniprot ID   A0A4P6X187
Organism   Hydrogenophaga pseudoflava strain DSM 1084     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4527210..4538829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPF_RS21795 (HPF_21665) - 4527987..4528766 (-) 780 WP_133157808.1 UPF0149 family protein -
  HPF_RS21800 (HPF_21670) - 4528828..4530447 (+) 1620 WP_133157809.1 FMN-binding glutamate synthase family protein -
  HPF_RS21805 (HPF_21675) - 4530451..4530888 (-) 438 WP_133157810.1 putative toxin-antitoxin system toxin component, PIN family -
  HPF_RS21810 (HPF_21680) - 4530885..4532213 (-) 1329 WP_133157811.1 THUMP domain-containing protein -
  HPF_RS21815 (HPF_21685) rpoN/rpoN1 4532210..4533829 (-) 1620 WP_133157812.1 RNA polymerase factor sigma-54 Machinery gene
  HPF_RS21820 (HPF_21690) lptB 4533842..4534591 (-) 750 WP_066156716.1 LPS export ABC transporter ATP-binding protein -
  HPF_RS21825 (HPF_21695) lptA 4534627..4535226 (-) 600 WP_066156719.1 lipopolysaccharide transport periplasmic protein LptA -
  HPF_RS21830 (HPF_21700) - 4535404..4536042 (-) 639 WP_066156722.1 thiol:disulfide interchange protein DsbA/DsbL -
  HPF_RS21835 (HPF_21705) - 4536113..4536781 (-) 669 WP_066156725.1 SPOR domain-containing protein -
  HPF_RS21840 (HPF_21710) argS 4536796..4538469 (-) 1674 WP_066156728.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 539 a.a.        Molecular weight: 59620.98 Da        Isoelectric Point: 4.5729

>NTDB_id=350303 HPF_RS21815 WP_133157812.1 4532210..4533829(-) (rpoN/rpoN1) [Hydrogenophaga pseudoflava strain DSM 1084]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLEMAQEVEQMLDENPFLERGEEVADREDFGVQTADAPVVAGEQLEEAAQP
VVELRSAEPDTRTTQADSADNSSDDVESRLDGAAPVEESWEGDGTVEMAPDDSEWGGDAPARNSGSGGDDDDTATAADLA
RVPVSLQDQLHEQARCLRLSDEDSAALFFLIESLNDDGYLEDSLHELARACWRLTAGRPADEEPRFDEVEAIEQRLGMAL
QWLQQMEPTGVGARDLGECLRLQIMELRNTPAARAALAICEQPLELMAKRDIKRLCAATGHDDETVRAALALIARLEPKP
GRRFVDVERNVVVPDVIVTRAGRGFKVSLNAEVMPRLRVHDVYANALRGRREAGGEAGHAAMQQRLQEARWFIKNIQQRF
DTILRVSSAIVERQRNFFMHGELAKRPLVLREIADELGLHESTISRVTTAKYMATPFGTYELKYFFGSSLGTETGGNASS
TAVRALLKQFIASEEPTKPLSDNQLSDLLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1620 bp        

>NTDB_id=350303 HPF_RS21815 WP_133157812.1 4532210..4533829(-) (rpoN/rpoN1) [Hydrogenophaga pseudoflava strain DSM 1084]
ATGAAGCAAGGTCTGTCGCTGCGGGTGTCGCAGCACCTCGCGCTGACGCCGCAACTGCAGCAGTCGATCCGCCTGTTGCA
GCTGTCCACGCTGGAGATGGCTCAGGAAGTCGAGCAGATGCTCGACGAGAACCCGTTCCTGGAACGGGGCGAGGAAGTGG
CCGATCGCGAAGATTTCGGCGTGCAGACCGCGGACGCGCCGGTGGTGGCCGGCGAGCAACTGGAAGAAGCGGCGCAGCCG
GTGGTCGAGCTGCGCAGCGCCGAGCCGGACACCCGCACCACCCAGGCCGACAGCGCGGACAACAGCAGCGACGACGTGGA
GAGCCGGCTGGACGGCGCCGCGCCGGTCGAAGAAAGCTGGGAAGGCGACGGCACCGTCGAGATGGCGCCCGACGACAGCG
AATGGGGCGGCGATGCACCCGCGCGCAACAGCGGTTCCGGCGGGGACGACGACGACACCGCCACGGCCGCCGACCTGGCC
CGTGTGCCGGTTAGCCTTCAGGACCAGCTGCACGAGCAGGCGCGCTGCCTGCGCCTGTCGGACGAGGACAGCGCGGCGCT
GTTTTTCCTGATCGAGTCGCTCAACGACGACGGGTATCTGGAGGACAGCCTGCACGAGCTGGCGCGCGCCTGCTGGCGCC
TGACCGCGGGCCGGCCGGCCGACGAAGAGCCGCGCTTCGACGAAGTCGAGGCCATTGAGCAGCGCCTGGGCATGGCCCTG
CAGTGGCTGCAGCAGATGGAGCCCACGGGCGTGGGCGCGCGCGACCTGGGCGAGTGCCTGCGGCTGCAGATCATGGAGCT
GCGCAACACGCCCGCCGCCCGCGCGGCGCTGGCGATCTGCGAGCAGCCGCTGGAGCTGATGGCCAAGCGCGACATCAAGC
GCCTGTGTGCGGCCACCGGGCACGACGACGAGACCGTGCGCGCCGCGCTGGCGCTGATCGCCCGCCTGGAGCCCAAGCCG
GGCCGGCGCTTCGTCGACGTCGAGCGCAACGTCGTGGTGCCCGACGTGATCGTCACCCGCGCCGGTCGCGGTTTCAAGGT
CTCGCTCAACGCCGAGGTGATGCCGCGCCTGCGCGTGCACGATGTCTATGCCAACGCCCTGCGGGGGCGGCGCGAGGCCG
GCGGCGAAGCGGGCCACGCGGCCATGCAGCAGCGGCTGCAGGAAGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTC
GACACCATCCTGCGCGTGTCCTCGGCCATCGTGGAGCGCCAGCGCAACTTCTTCATGCACGGCGAACTGGCCAAGCGCCC
GCTGGTGCTGCGCGAGATCGCCGACGAGCTGGGCTTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGG
CCACGCCGTTCGGCACCTACGAGCTCAAGTACTTTTTCGGCTCCAGCCTGGGAACGGAGACCGGCGGCAATGCGTCGAGC
ACGGCGGTGCGCGCCCTGCTCAAGCAGTTCATCGCGTCCGAAGAGCCGACCAAGCCGCTGTCCGACAACCAGCTCTCGGA
CCTGCTCAAGGAGCAGGGCATCGAGTGCGCGCGCCGCACGGTCGCGAAATACCGCGAGGCGCTGCGCATCGCGCCGGCGA
ACCTGAGAAAAGCGCTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(161-363)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(381-537)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.277

100

0.521

Multiple sequence alignment