Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   GJD94_RS19590 Genome accession   NZ_CP046003
Coordinates   3878555..3878995 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 1916D6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3873555..3883995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD94_RS19570 (GJD94_19560) aroP 3874553..3875926 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  GJD94_RS19575 (GJD94_19565) ampE 3875969..3876823 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GJD94_RS19580 (GJD94_19570) ampD 3876820..3877371 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GJD94_RS19585 (GJD94_19575) nadC 3877459..3878352 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GJD94_RS19590 (GJD94_19580) pilA 3878555..3878995 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  GJD94_RS19595 (GJD94_19585) pilB 3879005..3880390 (+) 1386 WP_021570596.1 type II secretion system protein GspE Machinery gene
  GJD94_RS19600 (GJD94_19590) hofC 3880380..3881582 (+) 1203 WP_000157266.1 protein transport protein HofC -
  GJD94_RS19605 (GJD94_19595) guaC 3881617..3882660 (-) 1044 WP_001217338.1 GMP reductase -
  GJD94_RS23685 - 3882816..3882860 (-) 45 WP_120795372.1 protein YacM -
  GJD94_RS19610 (GJD94_19600) coaE 3882885..3883505 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence


Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=349400 GJD94_RS19590 WP_000360904.1 3878555..3878995(+) (pilA) [Escherichia coli strain 1916D6]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=349400 GJD94_RS19590 WP_000360904.1 3878555..3878995(+) (pilA) [Escherichia coli strain 1916D6]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB P36647

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363


Multiple sequence alignment