Detailed information
Bioinformatically predicted
Overview
| Name | pilN | Type | Machinery gene |
| Locus tag | EZZ25_RS05300 | Genome accession | NZ_CP036499 |
| Coordinates | 1092853..1093437 (+) | Length | 194 a.a. |
| NCBI ID | WP_000902746.1 | Uniprot ID | - |
| Organism | Vibrio cholerae strain 20000 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Genomic Context
Location: 1087853..1098437
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EZZ25_RS05285 (EZZ25_05285) | oxyR | 1088259..1089152 (+) | 894 | WP_001914100.1 | DNA-binding transcriptional regulator OxyR | - |
| EZZ25_RS05290 (EZZ25_05290) | - | 1089227..1091704 (-) | 2478 | WP_165490517.1 | penicillin-binding protein 1A | - |
| EZZ25_RS05295 (EZZ25_05295) | pilM | 1091847..1092863 (+) | 1017 | WP_000520446.1 | type IV pilus assembly protein PilM | Machinery gene |
| EZZ25_RS05300 (EZZ25_05300) | pilN | 1092853..1093437 (+) | 585 | WP_000902746.1 | PilN domain-containing protein | Machinery gene |
| EZZ25_RS05305 (EZZ25_05305) | pilO | 1093430..1094020 (+) | 591 | WP_000157688.1 | type 4a pilus biogenesis protein PilO | Machinery gene |
| EZZ25_RS05310 (EZZ25_05310) | pilP | 1094010..1094528 (+) | 519 | WP_000792030.1 | pilus assembly protein PilP | Machinery gene |
| EZZ25_RS05315 (EZZ25_05315) | pilQ | 1094554..1096269 (+) | 1716 | WP_000788427.1 | type IV pilus secretin PilQ family protein | Machinery gene |
| EZZ25_RS05320 (EZZ25_05320) | aroK | 1096456..1096980 (+) | 525 | WP_000818616.1 | shikimate kinase AroK | - |
| EZZ25_RS05325 (EZZ25_05325) | aroB | 1097008..1098093 (+) | 1086 | WP_000439812.1 | 3-dehydroquinate synthase | - |
Sequence
Protein
Download Length: 194 a.a. Molecular weight: 22022.45 Da Isoelectric Point: 9.7294
>NTDB_id=349225 EZZ25_RS05300 WP_000902746.1 1092853..1093437(+) (pilN) [Vibrio cholerae strain 20000]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG
Nucleotide
Download Length: 585 bp
>NTDB_id=349225 EZZ25_RS05300 WP_000902746.1 1092853..1093437(+) (pilN) [Vibrio cholerae strain 20000]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATTTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAACGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACATAATGGCTAG
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATTTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAACGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACATAATGGCTAG
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilN | Vibrio cholerae strain A1552 |
99.485 |
100 |
0.995 |
| pilN | Vibrio campbellii strain DS40M4 |
60.452 |
91.237 |
0.552 |