Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EZZ25_RS05295 Genome accession   NZ_CP036499
Coordinates   1091847..1092863 (+) Length   338 a.a.
NCBI ID   WP_000520446.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1086847..1097863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS05280 (EZZ25_05280) - 1087388..1088119 (-) 732 WP_162796790.1 glutathione peroxidase -
  EZZ25_RS05285 (EZZ25_05285) oxyR 1088259..1089152 (+) 894 WP_001914100.1 DNA-binding transcriptional regulator OxyR -
  EZZ25_RS05290 (EZZ25_05290) - 1089227..1091704 (-) 2478 WP_165490517.1 penicillin-binding protein 1A -
  EZZ25_RS05295 (EZZ25_05295) pilM 1091847..1092863 (+) 1017 WP_000520446.1 type IV pilus assembly protein PilM Machinery gene
  EZZ25_RS05300 (EZZ25_05300) pilN 1092853..1093437 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  EZZ25_RS05305 (EZZ25_05305) pilO 1093430..1094020 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  EZZ25_RS05310 (EZZ25_05310) pilP 1094010..1094528 (+) 519 WP_000792030.1 pilus assembly protein PilP Machinery gene
  EZZ25_RS05315 (EZZ25_05315) pilQ 1094554..1096269 (+) 1716 WP_000788427.1 type IV pilus secretin PilQ family protein Machinery gene
  EZZ25_RS05320 (EZZ25_05320) aroK 1096456..1096980 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.45 Da        Isoelectric Point: 7.1127

>NTDB_id=349224 EZZ25_RS05295 WP_000520446.1 1091847..1092863(+) (pilM) [Vibrio cholerae strain 20000]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVQVTEKSLVRSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=349224 EZZ25_RS05295 WP_000520446.1 1091847..1092863(+) (pilM) [Vibrio cholerae strain 20000]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCCAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482

Multiple sequence alignment