Detailed information NTG0349089

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EZZ79_RS03940
• Genome accession: NZ_CP036493
• Coordinates: 891058..891930 (-)
• Length: 290 a.a.
• NCBI ID: WP_024664611.1
• Uniprot ID: -
• Organism: Pseudomonas syringae strain Psy33
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 886058..896930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EZZ79_RS03910 (EZZ79_04145)	-	886548..887177 (-)	630	WP_003406555.1	DUF1780 domain-containing protein	-
EZZ79_RS03915 (EZZ79_04150)	-	887304..887771 (+)	468	WP_024664607.1	hypothetical protein	-
EZZ79_RS03920 (EZZ79_04155)	-	888062..889534 (+)	1473	WP_024664608.1	tetratricopeptide repeat protein	-
EZZ79_RS03925 (EZZ79_04160)	-	889641..890099 (+)	459	WP_024664609.1	hypothetical protein	-
EZZ79_RS03930 (EZZ79_04165)	yacG	890232..890441 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
EZZ79_RS03935 (EZZ79_04170)	coaE	890438..891061 (-)	624	WP_024664610.1	dephospho-CoA kinase	-
EZZ79_RS03940 (EZZ79_04175)	pilD	891058..891930 (-)	873	WP_024664611.1	A24 family peptidase	Machinery gene
EZZ79_RS03945 (EZZ79_04180)	pilC	891932..893149 (-)	1218	WP_024664612.1	type II secretion system F family protein	Machinery gene
EZZ79_RS03950 (EZZ79_04185)	pilB	893152..894846 (-)	1695	WP_024664613.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EZZ79_RS03955 (EZZ79_04190)	-	895070..895498 (+)	429	WP_024664614.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
EZZ79_RS03960 (EZZ79_04195)	-	895966..896487 (+)	522	WP_024664615.1	glutathione binding-like protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31906.11 Da
• Isoelectric Point: 8.3734

>NTDB_id=349089 EZZ79_RS03940 WP_024664611.1 891058..891930(-) (pilD) [Pseudomonas syringae strain Psy33]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PLENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=349089 EZZ79_RS03940 WP_024664611.1 891058..891930(-) (pilD) [Pseudomonas syringae strain Psy33]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCAGAGAACTGCTCGGCC
TGCCTGCTGAACCCGATCAACCCGTCTTCAATCTGAACCGCCCCCGTTCCAGTTGCCCGCATTGCGCACACAAAATCCGC
CCATTGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGCAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCACTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCATACGTGGCCTGGCATTTCGGGTTCGGCTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTCACCTCGCTGAATGATGCGCTATGGGGGGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.004	97.586	0.517
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472